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2 Polish Journal of Applied Chemistry

2 2009

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Separation and characterization of active proteins from (rheumribes roots) which have hypoglycemic activity in normal and alloxan-induced diabetic mice

Saleh B. A., Sabir D. M., Hussin F. H. M.

Polish Journal of Applied Chemistry

2009

Vol. 53, nr 4

303-313

Hypoglycemic activity of aqueous extract and sequential solnent extract of medicago sativa (lucern) flower in normal and alloxan induced dlabetic mice

Sabir D. M., Saleh B. A., Hussain F. H. S., Raoof H., Tantawy A. S.

Polish Journal of Applied Chemistry

2009

Vol. 53, nr 4

295-302

Medicago Sativa (alfalfa) is used as a traditional plant treatment of diabetes mellitus. In the present study hypoglycemic effect of aqueous extract and sequential extraction by increasing polar solvents on alfalfa flower were investigated in both normal and alloxan-induced diabetic mice. It was observed that the aqueous extract of alfalfa flower possess significant blood-sugar lowering in alloxan induced diabetic mice. However, this extract did not show hypoglycemic action in healthy mice. By sequential extraction with many solvents, ethyl acetate fraction showed hypoglycemic activity. Glucose tolerance test was also improved in both normal and alloxan- induced diabetic mice. In addition, the active compounds were identified bv HPLC chromatography and their mechanism of actions as hypoglycemic agent were also studied against estimation of malonaldehyde in vivo.