Wyniki wyszukiwania - BazTech

1

Low-active high-density Noctiluca scintillans cells in surface seawater

Tada Kuninao, Asahi Toshimasa, Kitatsuji Saho, Nomura Mika, Yamaguchi Hitomi, Ichimi Kazuhiko

Oceanologia

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2020

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No. 62 (3)

402--407

EN

Noctiluca scintillans is an important member of the mesozooplankton in terms of biomass and production in the Seto Inland Sea, Japan. The densities and adenosine triphosphate (ATP) contents of N. scintillans cells were measured. Vertical profiles of N. scintillans cellular activity in the coastal water were determined and the ATP contents were high at middle layers, with a maximum depth of 10 m. ATP contents were low in the surface and lower layers. These results suggest that active N. scintillans cells in subsurface layers with low density play an important role in the coastal ecosystem, and high-density cells in the surface water are not active.

2

Simultaneous analysis of adenosine triphosphate and its metabolites in Saccharomyces Cerevisiae using RP-HPLC

Zhu P., Wang S., Wang J., Zhou L., Shi P.

Acta Chromatographica

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2016

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Vol. 28, no. 4

501--511

EN

In order to assess the contribution of adenosine triphosphate and its metabolites to the cellular metabolism process in Saccharomyces cerevisiae, it is very important to simultaneously determine the relative concentrations of ATP and its metabolites. In this study, a fast, simple reversed-phase high-performance liquid chromatography with high selectivity was developed to simultaneously measure adenosine triphosphate and its metabolites (adenosine diphosphate, adenosine monophosphate, and cyclic adenosine monophosphate) in yeast. The method was performed under the gradient grogram, and the detection was monitored at 254 nm. Analysis was achieved within 25 min. The four components can be detected with linear response over the concentration range from 1 to 100 mg L−1 with excellent correlation coefficients (r2) > 0.999. The recovery of the four analytes was 92.9%, 90.4%, 99.1%, and 105.1%, respectively. To demonstrate the good analysis of yeast samples, changes in the four adenine nucleotides levels caused by caloric restriction in yeast were determined. It is expected that the current method may contribute to further metabolomics and system biology investigations of yeast.

3

Bioluminescencyjny pomiar ATP - alternatywna metoda mikrobiologiczna w kontroli higieny

Ficek A., Michalski R.

Laboratorium - Przegląd Ogólnopolski

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2014

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nr 7-8

49--51

PL

Pomiar adenozynotrójfosforanu jest stosowany m.in. do określenia warunków sanitarnych w przemyśle spożywczym, mieszkalnictwie czy w hotelarstwie. W przeciwieństwie do czasochłonnych i pracochłonnych klasycznych metod mikrobiologicznych umożliwia on szybkie otrzymywanie wyników. Ponadto test można wykonywać w warunkach poligonowych, a uzyskane dane stanowią podstawę do podejmowania określonych działań naprawczych i zapobiegawczych.

EN

The measurement of adenosine triphosphate is used e.g. for an assessment of hygienic conditions in food industry, residential buildings and hotels. As opposed to time-consuming and laborious traditional microbial analysis, the measurement of ATP provides quick results. Moreover, the test can be performed in the field conditions and obtained results are the basis for decision-making with regards to adequate corrective and preventive actions.

4

Bioluminescencyjny pomiar ATP - podstawy teoretyczne i możliwości wykorzystania

Czaczyk K.

Laboratorium - Przegląd Ogólnopolski

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2002

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nr 2

18--20

PL

Wszystkie żywe komórki zawierają ATP (adenozynotrifosforan). Jest to nukleotyd zbudowany z adeniny, rybozy i trójfosforanu, którego akytwną formę stanowi kompleks z jonami metalu (magnez, mangan, kobalt, żelazo, nikiel lub cynk). ATP jest przenośnikiem energii swobodnej w większości procesów metabolicznych w komórce, takich jak synteza biocząsteczek, czy ich transport.