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1 Journal of Ecological Engineering

1 2019

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Isolation and Identification of Native Bacteria from Total Petroleum Hydrocarbon Polluted Soil in Wonocolo Public Oilfields, Indonesia

Sari Gina Lova, Trihadiningrum Yulinah, Ni’matuzahroh

Journal of Ecological Engineering

2019

Vol. 20, nr 8

60--64

The presented study concerns on isolation and identification of indigenous bacteria in total petroleum hydrocarbon (TPH) polluted soil. The composite TPH polluted soil was collected from Wonocolo public oilfields, Indonesia. Pour plate and plate count techniques were used to bacterial population analysis and enumeration, respectively. Two dominant bacterial colonies were isolated from 4.06x107 CFU/g population in polluted soil, then morphologically and biochemically were characterized using Microbact Identification Kits (MicrobactTM GNB12A and 12B). The identification of isolated bacteria was performed using Bergey’s Manual of Determinative Bacteriology. The results showed that the strains of bacteria are Bacillus sp. and B. cereus with probability of 72.00 and 77.00%, respectively. These strains potentially acted as biosurfactant producers and hydrocarbon degraders. Thus, biostimulation could be implemented to reduce the TPH levels in polluted soil at Wonocolo public oilfields.