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Antioxidant properties of Moringa oleifera and Withania somnifera extracts and their use in cosmetics for men

Jezierska Adrianna, Sykuła Anna

Biotechnology and Food Science

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2023

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Vol. 85, nr 1

34--42

EN

Nowadays, men are paying more and more attention to their appearance, and thus try to provide their skin with proper care so that it looks healthy and without signs of aging. In the present study, ashwagandha (Withania somnifera) and moringa oleifera (Moringa oleifera) were selected from the most popular adaptogens used in cosmetics, which are currently not commonly used in cosmetic preparations for men. The selected adaptogens were compared for their phytochemical and antioxidant properties to determine their skin care effects in cosmetics. The phytochemical content was evaluated through the determination of total phenolic content and in vitro antioxidant capacity was determined by DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS{2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)} free radical scavenging tests. The total phenolic contents 1.81±0.13 and 0.14±0.02 mg GAE/g extract were found to be present in Moringa oleifera and Withania somnifera glycol-water extracts, respectively. Among the two chosen extracts, Moringa oleifera exhibited significant free radical scavenging activity, ABTS (0.964 ± 0.021 mg TE/g extract) and DPPH (0.822 ± 0.004 mg TE/g extract). It can be concluded that Moringa oleifera extract has strong antioxidant properties than Withania somnifera extract.

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LC-MS/MS method for the quantification of withaferin-A in plant extracts of Withania spp.

Mirjalili M. H., Navarro A., Hernández L., Jáuregui O., Bonfill M.

Acta Chromatographica

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2013

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Vol. 25, no. 4

745--754

EN

The quantification of the main withanolide, withaferin-A, in plant extracts of Withania somnifera and Withania coagulans using high-performance liquid chromatography (HPLC)-mass spectrometry (ESI-MS/MS) is described. HPLC was used in combination with a triple quadrupole mass spectrometer in multiple reaction monitoring (MRM) mode. Additionally, mass exact determination of withaferin-A was achieved using a hybrid high-resolution mass spectrometer (Q-TOF). A combination of acetonitrile and water as the mobile phase using gradient elution was found to be successful in separating the compound of interest from the other main components. The limit of detection (LOD) in the plants was 6 ng/g and the total analysis time was 10 min.

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RP-HPLC analysis of withanolides in the flowers, leaves, and roots of Withania somnifera

Ali N. W, Abouzid S, Nasib A, Khan S., Qureshi J, Choudhary M. I

Acta Chromatographica

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2010

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Vol. 22, no. 3

473--480

EN

Ashwaghanda, Withania somnifera , is one of the most widely used herbs in Ayurvedic medicine. Leaves and roots are the traditionally used parts of the plant. An RP-HPLC method using a binary acetonitrile-water gradient containing 0.1% acetic acid has been developed for analysis of withaferin A. The method was validated in accordance with ICH guidelines and used for analysis of the withanolide content of the flowers, leaves, and roots of W. somnifera . The withanolide content was highest in the flowers.