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Immunomodulatory Effects of 900 MHz Magnetic Field Applied on Human Blood T Lymphocytes in vitro

Stankiewicz W., Samochocki Z., Kieliszek J., Sobiech J., Bartosiński A., Bodera P., Krawczyk A., Dąbrowski M. P.

Przegląd Elektrotechniczny

2011

R. 87, nr 12b

155--156

T lymphocytes were isolated from blood samples of 15 patients suffering from atopic dermatitis and 20 healthy donors. Cell cultures were exposed to the impact of 900 MHz electromagnetic field in the special anechoic chamber. The immunomodulatory effects of 900 MHz magnetic field on T lymphocytes were observed especially on cells isolated from patients suffering from atopic dermatitis

Limfocyty T zosta!y wyizolowane z krwi 20 zdrowych dawców i 15 chorych na atopowe zapalenie skóry. Hodowle komórkowe poddawane były dzia!aniu pola elektromagnetycznego o częstotliwości 900 MHz w specjalnej komorze bezechowej. Wyniki wskazują na korzystne immunomodulacyjne działanie 900 MHz PEM zw!aszcza na limfocyty T pochodzące od chorych na atopowe zapalenie skóry.

DNA damage in subpopulations of human lymphocytes irradiated with doses in the range of 0-1 Gy of X-radiation

Wojewódzka M., Machaj E. K., Goździk A., Iwaneńko T., Ołdak T., Kruszewski M., Pojda Z.

Nukleonika

2008

Vol. 53, No. 4

145-149

We compared three methods usually applied in biological dosimetry for estimation of radiation-induced DNA damage in human T and B lymphocytes: alkaline comet assay, micronucleus (MN) test and formation of histone gamma-H2AX foci. Human peripheral blood lymphocytes were fractionated using T cells and B cells isolation kits. Cells were irradiated with doses in the range of 0-1 Gy of X-rays. Induction of DNA damage was assessed by the standard alkaline comet assay, MN test and histone gammaH2AX foci immunofluorescence assay. Notwithstanding different end-points measured by the applied methods, all tests revealed a similar induction of DNA damage in B lymphocytes as compared with T lymphocytes. The results indicated that all three tests detect DNA damage with similar sensitivity, the lowest dose being approximately 0.3 Gy. The difference between irradiated and control cells was expressed as the ratio of the value obtained for irradiated cells (1 Gy) to that for control cells. The highest ratio was obtained for formation of gammaH2AX foci and was 6.2 for T and 13.8 for B lymphocytes, whereas those for comet assay and micronucleus test were 3.5; 3.6 and 5.6; 4.8, respectively.