Wyniki wyszukiwania - BazTech

Ograniczanie wyników

1 Acta Chromatographica

1 2013

Znaleziono wyników: 1

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: Salix matsudana

Sortuj według:

Ogranicz wyniki do:

Development and validation of a RP-HPLC method for simultaneous determination of salicin and eight flavonoids in leaves of Salix Matsudana Koidz

Li W., Shi L. L., Han L. Q., Zhang J.

Acta Chromatographica

2013

Vol. 25, no. 4

735--743

A reversed-phase high-performance liquid chromatographic method was developed for the first time to simultaneously determine salicin and eight flavonoids in leaves of Salix matsudana, that is salicin, luteolin-7-O-glucoside, myricetin, apigenin-3′-oxyethyl-7-O-glucoside, rutin, quercetin, luteolin, kaempferol and apigenin. The separation of these compounds was achieved on a reversed phase C18 column (250 × 4.6 mm, 5 μm), with linear gradient of methanol in 0.2% phosphoric acid solution with a flow rate of 1.0 mL/min with UV detection at 246 nm. The calibration curves for the determination of all analytes showed good linearity over the investigated ranges (r > 0.999). The % relative standard deviation (% RSD) values were less than 0.34%, and the recoveries were between 95.79% and 99.94%. The values of luteolin-7-O-glucoside, salicin, myricetin, apigenin-3′-oxyethyl-7-O-glucoside, rutin, quercetin, luteolin, kaempferol, and apigenin were 1.0 μg g-1, 20.0 μg g-1, 32.9 μg g-1, 2.0 μg g-1, 29.5 μg g-1, 6.0 μg g-1, 1.0 μg g-1, 3.5 μg g-1, and apigenin was not found in the sample. This developed method can be used for evaluating the quality of different plant materials.