Wyniki wyszukiwania - BazTech

Ograniczanie wyników

1 Journal of Ecological Engineering

1 2015

Powiadomienia systemowe

Sesja wygasła!

Znaleziono wyników: 1

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: SOS-gfp biosensor

Sortuj według:

Ogranicz wyniki do:

Potential applications of SOS-GFP biosensor to in vitro rapid screening of cytotoxic and genotoxic effect of anticancer and antidiabetic pharmacist residues in surface water

Matejczyk M., Rosochacki S. J.

Journal of Ecological Engineering

2015

Vol. 16, nr 1

116--121

Escherichia coli K-12 GFP-based bacterial biosensors allowed the detection of cytotoxic and genotoxic effect of anticancer drug– cyclophosphamide and antidiabetic drug – metformin in PBS buffer and surface water. Experimental data indicated that recA::gfpmut2 genetic system was sensitive to drugs and drugs mixture applied in experiment. RecA promoter was a good bioindicator in cytotoxic and genotoxic effect screening of cyclophosphamide, metformin and the mixture of the both drugs in PBS buffer and surface water. The results indicated that E. coli K-12 recA::gfp mut2 strain could be potentially useful for first-step screening of cytotoxic and genotoxic effect of anticancer and antidiabetic pharmacist residues in water. Next steps in research will include more experimental analysis to validate recA::gfpmut2 genetic system in E. coli K-12 on different anticancer drugs.