Wyniki wyszukiwania - BazTech

1

Using of UV lamps and UV robots for disinfection in the medical practice and in the hospital during the coronavirus epidemic

Krisch Henrik, Lelińska Katarzyna, Zhanghao Yao

Przegląd Techniczny : Gazeta Inżynierska

|

2020

|

nr 20-21

20--27

EN

In this article are presented different types of bactericidal / viricidal UV lamps and UV Robots and their effectiveness in the inactivation of bacteria, viruses, fungi, protozoa and mites. In particular it describes vacuum UV-V lamps and robots that can generate ozone. Ozon has the possibility to reach all objects inaccessible to direct radiation and it also destroys these microorganisms. The next type of bactericidal ozone-free UV-C lamps and UV-C robots, which shorten the disinfection time. All topics presented in the article combined with the use of UV lamps and UV robots are based on the results of laboratory tests in various countries (Germany, USA, China, South Korea, Denmark) that were applied to destroy SARS-CoV-2 corona viruses.

2

Efficient Computation of RNA Partition Functions Using McCaskill’s Algorithm

Zhao Chunchun, Sahni Sartaj

Annals of Computer Science and Information Systems

|

2020

|

Vol. 21

449--452

EN

We develop efficient single- and multi-core algorithms to compute partition functions for RNA sequences. Our algorithms, which are based on McCaskill's algorithm, are benchmarked against state-of-the-art fast algorithms obtained using the parallelizing source-to-source compilers PLUTO and TRACO. On our Intel I9 computational platform, our best single core algorithm takes up to 81.2% less time than the single core algorithm resulting from PLUTO, which is faster than that obtained from TRACO. Our best multi-core algorithm takes up to 84.7% less time than the multi-core algorithm obtained using TRACO when run with 20 threads (our I9 has 10 cores and supports hyperthreading); the TRACO multi-core algorithm is faster than the PLUTO one.

3

Wpływ dodatku kwasu deoksyrybonukleinowego (DNA) na właściwości mechaniczne żeli żelatynowych

Głazowska Joanna, Tylingo Robert, Bartoszek Agnieszka

Przemysł Chemiczny

|

2019

|

T. 98, nr 8

1224--1229

PL

Kwasy nukleinowe cieszą się coraz większym zainteresowaniem pod względem zastosowania ich w przemyśle farmaceutycznym, kosmetycznym oraz żywnościowym. Określono wpływ dodatku długo- i krótkołańcuchowych kwasów nukleinowych na właściwości mechaniczne żeli żelatynowych. Badania wykazały występowanie specyficznych interakcji pomiędzy żelatyną a kwasami nukleinowymi oraz pozwoliły na ocenę wpływu tych oddziaływań na twardość, kohezyjność, gumowatość oraz lepkość żeli.

EN

Two pork gelatin solns. were prepd. in deionized H2O (6.66 and 10% by mass) at 65°C and then gelled at 10°C for 24 h. The well-defined portions of the gelatin were added to solns. of two types of nucleic acids (short- and long-chain, gDNA and fDNA, resp.) dissoloved in a buffer (Tris-HCl and EDTA, pH 7.5) and mixed with deionized H2O to obtain concns. in the range of 0.001–0.1% by mass. The hardness, cohesiveness, elasticity, gumminess and viscosity at 40°C of obtained gelatin gels were detd. The mech. parameters of gelatin gels were deteriorated in the presence of gDNA but an improvement of these parameters was obsd. for the gel cong. 10% gelatin and 0.1% fDNA.

4

MicroRNAs in atherosclerosis: altered expression and diagnostic potential

Bogucka-Kocka A., Zalewski D., Ruszel K., Kocki J.

Engineering of Biomaterials

|

2018

|

Vol. 21, no. 148

76

5

Amplifikacja kwasów nukleinowych w warunkach izotermicznych - cz. I

Hołysz M., Hołysz H., Burzyński A.

Laboratorium - Przegląd Ogólnopolski

|

2015

|

nr 9-10

59--64

PL

Amplifikacja kwasów nukleinowych w warunkach izotermicznych stanowi ciekawą alternatywę wobec technik tradycyjnie wykorzystywanych w badaniach naukowych i w diagnostyce. W ciągu ostatnich kilkunastu lat opracowano wiele różnych metod powielania DNA i RNA w stałej temperaturze. Techniki te nie ustępują pod względem wielu parametrów klasycznej reakcji PCR, nawet technice real-time PCR, a niekiedy nawet je przewyższają. Cele niniejszego artykułu to: prezentacja technik izotermicznej amplifikacji kwasów nukleinowych, wyjaśnienie zasady ich działania oraz prezentacja produktów diagnostycznych działających w oparciu o te techniki.

EN

Amplification of nucleic acids in isothermal conditions is an interesting alternative for techniques traditionally used in research and diagnostics. For the last several years many different methods of DNA and RNA replication in constant temperature have been developed. These techniques does not leg behind, in terms of many parameters, the classical PCR, and even real-time PCR technique, and sometimes they are even better. The aim of the article is to present the techniques of isothermal amplification of nucleic acids, explain their basic principles and present diagnostic products operating based on these techniques.

6

Amplifikacja kwasów nukleinowych w warunkach izotermicznych - cz. II

Hołysz M., Hołysz H., Burzyński A.

Laboratorium - Przegląd Ogólnopolski

|

2015

|

nr 11-12

43--49

PL

W ofercie wielu firm na całym świecie znajdziemy produkty działające w oparciu o amplifikację kwasów nukleinowych w warunkach izotermicznych. Wśród nich znajdują się zestawy odczynników przeznaczone do badań naukowych, a także szeroka gama testów przeznaczonych głównie do diagnostyki wirusologicznej i bakteriologicznej. Celem niniejszego artykułu jest wyjaśnienie zasady działania poszczególnych technik izotermicznej amplifikacji kwasów nukleinowych, a także prezentacja produktów diagnostycznych działających w oparciu o te techniki.

EN

Many companies around the world have in offer products acting on the basis of nucleic acid amplification under isothermal conditions. Among them are reagent kits for scientific research and a wide range of tests intended primarily for the diagnosis of viral and bacteriological. The purpose of this article is to explain the principles of individual techniques isothermal nucleic acid amplification, as well as the presentation of operating diagnostic products based on these techniques.

7

Zastosowania „click chemistry” w modyfikacjach nukleozydów i oligonukleotydów

Gładysz M., Milecki J.

Wiadomości Chemiczne

|

2014

|

[Z] 68, 7-8

617--643

EN

Since the year 2001 new ideology of clean and simple synthesis in organic chemistry has been established. The outstanding scientists Meldal and Sharpless presented their concepts of Click Chemistry. Among the reactions chosen for this concept the reaction of Copper(I) Catalyzed Alkyne-Azide Cycloaddition (CuAAC) became the most popular one. It is the basis of syntheses employed for building blocks synthesis in medicinal chemistry and material science. Libraries of potentially pharmacologically active anticancer and antivirus compounds possessing neutral triazol linkage could be easily obtained. Remarkable efficiency of CuAAC reaction influenced on DNA- and RNAbased synthesis of novel oligonucleotides derivatives. Many of nucleic acid molecular modifications found applications in enzymatic transformation, nucleic acid hybridization, molecular tagging and gene silencing. The CuAAC reaction allows for introducing modifications into practically every region of nucleoside/nucleotide/ oligonucleotide. This includes versatile modifications of the base moiety both aiming at the base pairing ability or specific labeling of the nucleoside unit. Different conjugates (bio-, fluorescent-, affinity- or spin labels) are being attached to the base part of the nucleic acid taking advantage of the presence of azide or alkyne substituents, which can be installed without great difficulty. Labeling at the sugar part of the nucleoside can be realized at the position 2’, 3’ or 5’, the latter two giving rise to the end-labeled oligonucleotides and the 2’ position serving as the attachment point for labeling inside the oligonucleotide chain. These kind of nucleic acid modifications are very promising. Versatility of CuAAC reactions is demonstrated by numerous examples of introducing modifications into practically every reactive site of the nucleotide/oligonucleotide molecule. The review systematically presents application of the “click” technique for modification of nitrogenous base, sugar or pseudosugar moiety or phosphorus center. Possibility of creating new kind of chain linkage, devoid of negative charge and nuclease resistant is also shown. This allows to design a new class of nucleic acid analogues, similar in its DNA-mimicking properties to PNA’s.

8

Eksploracja danych genetycznych bazy GenBank z wykorzystaniem usług sieciowych

Siążnik A., Małysiak-Mrozek B., Mrozek D.

Studia Informatica

|

2011

|

Vol. 32, nr 3B

35-51

PL

National Center for Biotechnology Information (NCBI) gromadzi ogromne liczby danych opisujących różne organizmy biologiczne na wiele różnych sposobów. Dane te są przechowywane we właściwych bazach danych, zarządzanych przez NCBI. Baza danych GenBank jest jedną z najbardziej znanych na świecie baz NCBI przechowujących dziesiątki milionów sekwencji nukleotydowych DNA i RNA. W niniejszym artykule przedstawiono autorski system eksploracji danych genetycznych bazy GenBank. System search GenBank pozwala nie tylko wyszukiwać i przeglądać dane biologiczne bazy GenBank, ale także łączyć znalezione wpisy bazy GenBank z danymi w innych bazach danych NCBI, dając w ten sposób możliwość międzybazowej eksploracji danych.

EN

National Center for Biotechnology Information (NCBI) collects huge amounts of data describing various biological organisms in several ways. These data are stored in appropriate databases, managed by the NCBI. GenBank is one of the world's most famous NCBI database storing tens of millions of nucleotide sequences of DNA and RNA. In this article, we present a new system designed to explore genetic data in the GenBank database. The search GenBank system not only allows to search and browse biological data in the GenBank, but also combine the GenBank database entries with items in other NCBI databases. Therefore, the search GenBank provides the cross-database exploration possibilities.

9

Spektroskopia NMR w badaniach strukturalnych kwasów nukleinowych. Część 1.

Gdaniec Z.

Wiadomości Chemiczne

|

2005

|

[Z] 59, 1-2

47--65

EN

NMR spectroscopy is a powerful method that allows detetmination of the structure and dynamics of nucleic acids and their complexes in solution with atomic resolution. A major breakthrough in the structure determination of nucleic acids by NMR was introduction of advanced and efficient methods for the labeling of RNA and DNA with13C and 15N and development of multidimensional, heteronuclear NMR techniques analogous to those used in protein NMR spectroscopy. The resonance assignment is a crucial step in the NMR study. A spectrum must be assigned before useful structural information can be extracted. The assignment of RNA is considerably more difficult than for DNA of similar size. This is mainly due to the much narrower spectral dispersion of the H2', H3', H4' and H'/5 " ribose protons relative to DNA. The methodology for sequential assignment of nucleic acids via lomonuclear NMR techniques relies on the assumption of helical structure and therefore fails in the case of nonhelical structures, that is typical of RNA. Development of 13C/15N labeling techniques has afforded heteronuclear multidementional experiments that utilize the favorable properties of 13C and 15N nuclei such as large one-and two-bond heteronuclear scalar coupling constants and large chemical shift dispersion. These experiments provide increased sensitivity of double and triple resonance experiments and help in overcoming the problem of severe spectral overlap. Progress in novel NMR methods stimulated also a design of experiments for conformationindependent sequential assignment. In nucleic acids, experiments that correlate base resonances among themselves as well as with sugar resonances allow unambiguous spectral assignment for the structures, where the conventional NOE-based methods may not be applied. Assignments of highly overlapped sugar resonances are facilitated enormously by the application of correlated experiments based on 13C-13C transfer. Additionally, triple resonance experiments allow correlation of neighboring nucleotides through the phosphodiester backbone. The arsenal of existing methods in structure calculations of nuclcic acids by NMR spectroscopy has recently been extended. For example, NMR methods have been developed to detect and measure scalar couplings via hydrogen bonds. The information about hydrogen bonds provides very useful restraints for structural determination, especially in case of noncanonical motifs. Furthermore, the use of methods that introduce anisotropic environments for nucleic acids in solution allows the measurement of residual dipolar couplings (RDC). RDCs yield orientation, rather than distance-based constraints. The RDCs contain global structural information on nucleic acids that cannot be obtained by standard solution NMR techniques. These constraints can both improve the local structure of nucleic acids and provide novel data on the global structure. Another NMR technique, TROSY has been introduced to effectively suppress transverse relaxation of 1H-15N and 1H-13C moieties. TROSY selects exclusively the narrow line of a 1H-15N doublet or 1H-13C multiplet, yielding improved spectral resolution and increased sensitivity of NMR experiment. Recent advances in solution NMR techniques provide tools for structural studies of large (> 30 residues) nucleic acids molecules.