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Selection of batch process conditions for microbiologicalproduction of lactic acid using waste whey

Lech Magdalena, Trusek Anna

Chemical and Process Engineering

2019

Vol. 40, nr 1

67-–76

The research was focused on the selection of the best conditions for the lactic acid production.As the organic source diluted waste whey was used. Two facultative anaerobic bacteria strains wereexamined:Lactobacillus rhamnosusandLactococcus lactis. The neeed of anaerobic conditions as wellas mineral supplementation of cultivation were investigated. It turned out that the oxidation was not thekey parameter, but cultivation medium needed a supplementation for higher process efficiency. Finally,Lactobacillus rhamnosusstrain was selected, for which LA production was app. 45% higher than forLc. lactis. On the other hand,Lactobacillus rhamnosuswas active at higher lactose concentration, thuswaste whey needed to be less diluted. Additionally, high values of product/substrate yield coefficientmake the process very efficient.