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Free fatty acids and a high initial amount of biomass in the medium increase the synthesis of mannosylerythritol lipids by Pseudozyma antarctica

Dzięgielewska E., Adamczak M.

Environmental Biotechnology

2013

Vol. 9, No. 1

14--18

It was shown that the addition of lipase to a YPG cultivation medium containing 5% (v/v) of rapeseed oil increased the yield of mannosylerythritol lipids synthesized by P. antarctica from 12.69 to 26.97g·L-1 compared to the control culture. As a renewable resource of free fatty acids for P. antarctica cultivation, post-refining fatty acids (Post-FFA) were used and after 192h a 85.43g·L-1 of biosurfactant was obtained. The experiments indicated that the biosurfactant synthesis could depend on carbon source availability and biomass concentration in the cultivation medium. The supplementation of the medium after 8 days of cultivation (fed-batch culture), with glucose to 2% (w/v) and Post-FFA to 20% (v/v), did not increase the concentration of the biosurfactant in the medium but did increase the total amount of the product from about 170 to 232g. By increasing the initial concentration of the biomass to 500g·L-1, the concentration of biosurfactant was increased to 120.50g·L-1 after 168h.

Carbon-source-dependent synthesis and composition of biosurfactant synthesized by Pseudozyma antarctica

Bednarski W., Narwojsz M., Adamczak M., Nawotka R.

Environmental Biotechnology

2006

Vol. 2, No. 1

31-36

Pseudozyma antarctica (formerly Candida antarctica) ATCC 28323 was cultivated in preselected conditions in a medium containing glucose and products of enzymatically hydrolyzed lactose in milk permeate (after ultrafiltration) supplemented with the waste fats (fish, pork and post-refining fatty acids) to induce and intensify biosurfactant synthesis. The yield (12.0-26.7*g/dm3) of biosurfactant synthesis and the fatty acid composition of its hydrophobic fractions depended on carbon sources, i.e. waste fats listed above which have been added to the medium. The obtained results indicate that the fatty acids from the waste fats used seemed to be directly incorporated in the mannosylerithritol lipids. The main fatty acids present in biosurfactant were C16:0, C18:0, C18:1,C18:2.