Wyniki wyszukiwania - BazTech

1

Sensitivity and dynamic phase response to thermal radiation of a polarization-maintaining fiber

Kyselak M., Dvorak F., Maschke J., Vlcek C.

Optica Applicata

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2018

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Vol. 48, nr 2

249--261

EN

This paper deals with the phase shift development between two polarization modes in birefringent fiber, caused by body heat transfer of different temperatures. The aim is to analyze sensitivity and dynamic behaviors, which are significant when optical fiber is used as a sensor of temperature field disturbance. The analysis is based on the values measured during thermal exposure of a section of type PANDA birefringent optical fiber to heat emitted by an exposure body of chosen temperature, placed at a defined distance. The effect of heat transfer through conduction and convection is suppressed by shielding with plastic wraps. The analyzed results, the Stokes parameters and subsequent phase shift, are measured by the polarimeter. The paper follows up on previously published measurement results.

2

Short review on atmospheric aerosols source apportionment methods

Szramowiat K., Styszko K., Gołaś J.

Challenges of Modern Technology

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2016

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Vol. 7, no. 2

7--13

EN

Appropriate understanding of particulate matter emission sources is required to properly establish the policies aiming at the aerosols emitters’ elimi nation. The article provides a short review on atmospheric aerosols source apportionment methods using three computing models.

3

Mass spectrometry based analysis of erythrocyte membrane associated proteins in chronic myeloid leukemia patients in Sri Lanka

Kottahachchi D. U., Ariyaratne T. R., Jayasekera G. A. U.

International Letters of Chemistry, Physics and Astronomy

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2014

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Vol. 19, iss. 1

74--86

EN

The research reported in this paper was conducted to analyze erythrocyte membrane associated proteins (ERMBPs) of some of chronic myeloid leukemia (CML) patients and selected individuals of Sri Lanka employing one dimensional sodium dodecyl sulphate poly acrylamide gel electrophoresis (1D-SDS-PAGE) combined with matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI–TOF-MS). Erythrocyte membranes from blood were isolated by osmotic lysis, centrifugation and washings. ERMBPs were separated on 1D-SDS-PAGE, visualized by silver staining and the separated protein bands dissected out from the gel and were subjected to digestion by proteolytic enzyme, trypsin, and the resulting peptide mixture was analyzed by MALDI-TOF-MS. Resulting experimental peptide mass values were analyzed by peptide mass fingerprint (PMF) technique. From this analysis 10 ERMBPs including α and β spectrin, ankyrin, band 3, band 4.1, band 4.2, band 7, dematin, actin, 55 KDa erythrocyte membrane protein were identified accurately with their primary structure information. The study was able to provide some evidence for Cathepsin associated cleavage of Band 3 anion transport protein in CML patients reported previously. In addition we were able to detect changes in gel bands between healthy controls and CML patients around the area of 20 kDa in the 1 D-SDS-PAGE. It was identified as nuclear protein Dbf 4 related factor 1 isoform 2. Although erythrocytes are devoid of nuclei, such unexpected nuclear proteins have been identified in previous research. We were successful in identifying several human ERMBPs with available resources. As the identified proteins were known to be related to pathology of some of the hematological diseases, this methodology could be extended to detect the protein changes in erythrocyte membrane protein associated diseases. Therefore, this initial research would at some point lead to discovery of biomarkers to these hematological diseases in Sri Lanka.

4

Przełomowe techniki wykrywania biomarkerów białkowych

Meyer M., Ketterlinus R., Deiniger S.- O., Ebert M., Roecken C.

Laboratorium - Przegląd Ogólnopolski

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2006

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nr 8-9

49--50

PL

Obecnie opracowywane są nowe zastosowania spektrometrii mas w proteomice, mające szansę w najbliższej przyszłości na wdrożenie w laboratoriach klinicznych. Pośród nich dwie bardzo obiecujące techniki już wzbudziły zainteresowanie w kręgach zajmujących się badaniami klinicznymi, a mianowicie profilowanie biomarkerów techniką MALDI MS oraz obrazowanie MALDI (MALDI Imaging).

EN

New mass spectrometric (MS) proteomics technologies are currently under investigation which have the potential to reach the clinical laboratory in the near future. Of these two very promising techniques have already gained much interest in the clinical research community, namely biomarker profiling by MALDI MS and MALDI imaging technology.