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1 Polish Journal of Chemistry

1 2004

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Sol-Gel Immobilization of Aldehyde Dehydrogenase and NAD+ on Screen-Printed Electrodes for Designing of Amperometric Acetaldehyde Biosensor

Noguer T., Szydłowska D., Marty J., Trojanowicz M.

Polish Journal of Chemistry

2004

Vol. 78 / nr 9

1679-1689

The stability of response of amperometric biosensors based on entrapment of aldehyde dehydrogenase (AlDH) and nicotinamide adenine dinucleotide (NAD+) in a sol-gel matrix on the surface of screen-printed electrodes modified with the mediator Meldola Blue-Reinecke salt was studied. The operational stability of biosensors was tested using acetaldehyde as substrate by applying a potential of -150 mV vs. pseudo Ag/AgCl screen-printed electrodes. The type and ratio of silicium alkoxides precursors, the amounts of AlDH and NAD+, as well as the influence of drying and storage temperature on the stability of electrodes were examined. Among the tested biosensors, the most stable devices were those incorporating a sol-gel layer containing both AlDH and NAD+, which was covered by an additional sol-gel layer in order to avoid the co-enzyme leaching.