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HR TEM examinations of nanodiamond particles for biomedical application

Mitura K.

Journal of Achievements in Materials and Manufacturing Engineering

2009

Vol. 37, nr 2

317-322

Purpose: The aim of the study is an analysis of a different type of nano-diamond powders on HR TEM (material characterisation, the normal distribution of grain size). These nano-powders were used in many biological researches and each of them had a special biological activity. Comparison between the material and biological properties of diamond powders answered many questions about the chemical and physical reactions on a boundary: diamond surface – living organism. Design/methodology/approach: In this work we used cytotoxicity assays using MTT test and HR TEM examinations. We examined the nanodiamond particles manufactured by detonation method, RF/MW PACVD method, RF PACVD method, pure diamond from De Beers Company and graphite powder as a control. Findings: In this subject the material characterisation of diamond powders are known but we examined a different type of powders and we manufactured some of these nanopowders in our laboratory. Practical implications: Nanocrystalline Diamond Coatings have many applications in various fields of medicine. Nanodiamond Particles are an extended surface of diamond powder. Biological research with endothelial and lung cancer cells are the introduction to application in human’s treatment. Originality/value: There are in vitro examinations with bioactive biomaterial. Nanodiamond particles have a very high bioactivity on cell level and inhibit cancerogenesis.

Pozaustrojowe badania nad toksycznym oddziaływaniem kopolimeru PLGA z dodatkiem hydroksyapatytu na ludzkie osteoblasty linii hFOB 1.19

Cieślik M., Mertas A., Morawska-Chochół A., Orlicki R., Owczarek A., Król W.

Engineering of Biomaterials

2009

Vol. 12, no. 89-91

97--102

W celu biologicznej oceny w warunkach in vitro kopolimeru glikolidu z laktydem z dodatkiem hydroksyapatytu (PLGA+HA) dokonano oceny stopnia jego cytotoksyczności względem ludzkich osteoblastów linii hFOB 1.19. Wykonano pomiar aktywności dehydrogenazy mitochondrialnej (test MTT) oraz dehydrogenazy mleczanowej (test LDH) po 24 i 48 godzinach kontaktu komórek z ekstraktem uzyskanym poprzez 8-dniową inkubację kompozytu w medium do hodowli osteoblastów. Kontaktowano ponadto badany materiał bezpośrednio z komórkami kościotwórczymi, a stopień cytotoksyczności oceniano po 24, 48 i 72 godzinach stosując w tym celu test LDH. W obu metodach badany materiał nie wpływał w sposób toksyczny na ludzkie osteoblasty.

In order to evaluate lactide-glycolide co-polymer with admixture of hydroxyapatite (PLGA+HA) from biological point of view in vitro conditions, its level of toxicity for human osteoblasts line hFOB 1.19 was assessed. The activity of mitochondrial dehydrogenase and lactate dehydrogenase was measured (MTT test and LDH test respectively) after 24 and 48 hours of the cells’ contact with the extract obtained through 8-day incubation of the composite in osteoblasts cultivation medium. Apart from that the material examined was contacted with bone-forming cells and its degree of toxicity was assessed after 24, 48 and 72 hours using LDH test. In both methods the material under examination did not have any toxic influence upon human osteoblasts.