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2 2019

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Electromagnetic field as an environmental factor affecting MCF-7 cell line in vitro

Koziorowska Anna, Kozioł Katarzyna, Hajduga Ewelina, Romerowicz-Misielak Maria

Przegląd Elektrotechniczny

2019

R. 95, nr 5

5--8

The purpose of the experiment was to obtain a response whether the electromagnetic field at the frequency of 50 Hz and magnetic induction of 2.5 mT affect the viability, proliferation and ability to migrate of MCF-7 cell line(the human breast adenocarcinoma cells) The viability of cells was assessed by trypan blue staining. The degree of cells proliferation was determined based on the cells density in culture, and the cells' ability to migrate was determined using plate clone assay and scratch adhesion test..The obtained results indicate the different effect of the EMF with the frequency of 50 Hz and magnetic induction of 2.5 mT on the viability, proliferation and the ability to migrate of the MCF-7 cell line in a manner depending on the time of exposure during the day (30 min, 10 min) as well as number of EMF exposure days (3 days, 6 days, 9 days).

Celem doświadczenia było uzyskanie odpowiedzi czy pole elektromagnetyczne o częstotliwości 50 Hz i indukcji magnetycznej 2,5 mT wpływa na żywotność, proliferację oraz zdolność do migracji komórek linii MCF-7 (komórki ludzkiego gruczolaka piersi). Przeżywalność komórek oceniano za pomocą barwienia błękitem trypanu. Stopień proliferacji komórek określano na podstawie gęstości komórek w hodowli a, zdolność komórek do migracji ustalano wykorzystując plate clone assay oraz scratch adhesion test. Otrzymane wyniki wskazują na różny wpływ pola elektromagnetycznego (50 Hz, 2.5 mT) na żywotność, proliferację i zdolność do migracji linii komórkowej MCF-7 w sposób zależny od czasu ekspozycji w ciągu dnia (30 minut, 10 minut), jak również od liczby dni ekspozycji (3 dni, 6 dni, 9 dni).

Study the Anti-MUC1 antibody-based iron oxide nanoparticles on three-dimension spheroid and breast cancer (MCF-7) cell imaging

Moradi Khaniabadi Pegah, Shahbazi-Gahrouei Daryoush, Shah Abdul Majid Amin Malik, Moradi Khaniabadi Bita

Polish Journal of Medical Physics and Engineering

2019

Vol. 25, Iss. 2

69--77

Non-invasive methods for breast cancer detection in early stages may help to increase the survival rate of patients. This study aimed to evaluate the application of Anti-MUC1 antibody-based iron oxide nanoparticle (SPIONs-C595) which was assessed in vivo as a molecular imaging probe for breast cancer (MCF-7) detection using MRI. Nine groups of female NRC NU/Nu mice (each group of 3), 6 to 8 weeks old were used and MCF-7 cells were injected subcutaneously into both flanks of nude mice. After two weeks the mice received an intravenous injection of different concentrations of SPIONs-C595. The uptake ability of SPIONs-C595 on three-dimension (3D) macrostructure is exploited a modified hanging drop method using Prussian blue for MCF-7 cells. The iron content was measured in liver, kidney, spleen, and tumor. The MR imaging features and biodistribution of nanoprobe was also investigated. The MR images obtained from digested tumor after nanoprobe administration in different time-period revealed that enhancement of T1 and T2 relaxation time. Moreover, the storage stability test was shown great application and no sedimentation of nanoparticles within two months storage at 4°C. Additionally, great validation of SPIONs-C595 on the 3D spheroid of MCF-7 was observed. The biodistribution analysis showed that iron content of the spleen was more than the other studied organs. These results highlighted the feasibility of an in-vivo model for detection of breast cancer MUC1 expression. Current researches are ongoing to further enhancement of relaxation times for classification of MUC1 status using clinical specimens.