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Minimal Conditions to Degrade Low Density Polyethylene by Aspergillus terreus and niger

Sáenz Melina, Borodulina Tamara, Diaz Linda, Banchon Carlos

Journal of Ecological Engineering

2019

Vol. 20, nr 6

44--51

Plastics pollution is a major worldwide concern because there is strong evidence about marine influence on the human trophic chain. Thus, we experimented with the biodegradation of low density polyethylene (LDPE) by A. niger and A. terreus in order to increase the degradation rate without any co-substrate or photothermal treatment. Our contribution is to show how to degrade LDPE under minimum nutritional conditions using both LDPE and sucrose as carbon sources. Up to 30% weight loss was obtained by A. niger and A. terreus which were isolated from an Ecuadorian mangrove. The evidence of cracks and biomass growth on the LDPE surface samples showed the potential of both fungi species to operate under low nutrient concentrations. The outlook of the present work was focused on understanding the fungi survival under minimal conditions.

Laccase concentration by foam fractionation of Cerrena unicolor and Pleurotus sapidus culture supernatants

Blatkiewicz M., Antecka A., Górak A., Ledakowicz S.

Chemical and Process Engineering

2017

Vol. 38, nr 3

455--464

Foam fractionation process for concentration of laccases from two Basidiomycete strains under different process conditions was investigated. Culture supernatants of Cerrena unicolor and Pleurotus sapidus containing active laccase were used with and without surfactant additives. Two surfactants: cationic cetrimonium bromide (CTAB) and non-ionic Polysorbate 80 were applied in the range from 0.2 mM to 1.5 mM. The pH levels ranging from 3 to 10 were examined with particular attention to pH=4, which is close to the pI of the enzymes. Results show that the source of the enzyme is significant in terms of partitioning efficiency in a foam fractionation process. Laccase from Cerrena unicolor showed the best activity partitioning coefficients between foamate and retentate of almost 200 with yields reaching 50% for pH 7.5 and concentration of CTAB cCTAB = 0.5 mM, whereas laccase from Pleurotus sapidus showed partitioning coefficients of up to 8 with 25% yield for pH 4 and cCTAB = 0.5 mM.