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Response surface methodology for optimization studies of hydro-distillation of essential oil from pixie mandarin (Citrus reticulata Blanco) peels

Tan Phat Dao, Ngo Thi Cam Quyen, Tran Thi Yen Nhi, Chi Cuong Nguyen, Trung Thanh Nguyen, Xuan Tien Le

Polish Journal of Chemical Technology

2021

Vol. 23, nr 4

26--34

Essential oil extraction technique from mandarin pixie peels by hydro-distillation is optimized by respon-se surface methodology (RSM). Mathematical techniques were used in experimental design to evaluate the impacts of factors that affect the extraction process and improve the yield of the extraction process. A central mixed design based on infl uencing variables such as water ratio (3–5 mL/g), temperature (110–130° C) and extraction time (90–150 min) was adopted with essential oil yield as the target func-tion. Correlation analysis of the mathematical regression model showed that the quadratic polynomial model can be used to optimize hydro-distillation of pixie mandarin oil. The results showed that under the optimum extraction conditions, the highest quantity of essential oils was achieved (7.28 mL/100 g materials). In terms of statistical analysis, the signifi cance levels (p-value <0.05) of the model showed that the experimental results had a good impact between factors. The coeffi cient of determination indicating the match between the experimental value and the predicted value of the model was high (R2>0.9). The chemical composition of the essential oil was analyzed by Gas Chromatography-Mass Spectrometry, revealing the dominance of limonene content (97.667%), which implies that the essential oil of pixie mandarin could be an alternative source of limonene.