Wyniki wyszukiwania - BazTech

Ograniczanie wyników

2 Nukleonika

Powiadomienia systemowe

Sesja wygasła!

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych: HYNIC

Sortuj według:

Ogranicz wyniki do:

In - house preparation and evaluation of Tc-99m-HIG kit for detection of infection in AIDS patients

Bakir M. A., Mansour R., Mohammad A., Habil K., Yassine T.

Nukleonika

2012

Vol. 57, No. 1

117-124

99mTc-HIG can be used for early detection of inflammation and infection foci especially in immunodeficiency patients. The present study involves in-house preparation and evaluation of human immunoglobulin-hydrazinonicotinic acid (HIG-HYNIC) kit to be labeled with 99mTc. In this indirect labeling method, the structure and biological activity of the protein can be maintained. The kit consists of two vials A and B, the vial A contains 2 mg of HIG-HYNIC, and the vial B contains 10 mg of tricine and 1 mg of stannous chloride dihydrate (SnCl2.2H2O). The results revealed that the lyophilized kits were stable for at least 10 months, and showed a high quality from the points of physical, chemical, radiochemical and biological purities, and its validity for human injection. The labeling yield of HIG-HYNIC compound exceeded 98%. The application of the prepared kit was experimentally illustrated in animal models with artificially induced inflammatory lesions after E. coli injection into rat leg muscle. The biodistribution and imaging studies in rats have shown good localization in infected muscle (T) in comparison to the normal muscle (M) 4 and 24 h after injection. Ratios of 17 and 18 were achieved 4 and 24 h post injection, respectively. The results of biodistribution of 99mTc-HIG in human and initial clinical evaluation show that this agent is a good radiopharmaceutical for infection imaging.

Preparation and biodistribution of 99mTc-IgG-HYNIC in normal rats

Rajabifar S., Akhlaghi M., Jalilian A. R., Bolourinovin F., Maashkar B., Talebimehrdar M., Ghafouri M.

Nukleonika

2009

Vol. 54, No. 4

279-284

Human gamma globulin can be labeled by a direct or indirect method of radiotracer incorporated in a protein molecule. In this indirect method hydrazinonicotinic acid (HYNIC) is used which saves the structure and biological activity of the protein. Our goal was the efficient labeling of the human gamma globulin and evaluation of its biodistribution in different organs which can be used on experimentally induced infection causing inflammation. Immune globulin is mixed with s-hynic and IgG-hynic is developed using sidle A-lyzer and stored at –20°C which can be used at least for six months and then Sn-tricine kit is prepared which is used for 99mTc labeling. Efficiency of 99mTc-IgG-hynic labeling at pH 6.4 was very much dependent on ligand (hynic) and coligand (tricine) presence in the reaction mixture. Radiochemical purity was more than 90% in the kits prepared. Serum stability study showed no decomposition of 99mTc from the complex. The biodistribution studies showed the highest percentage ID/organ in the blood, liver and kidney, respectively. A human gamma globulin was successfully labeled through hynic to 99mTc by an indirect method with high radiochemical purity.