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Application of LC and HPTLC-densitometry for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride and cyanocobalamine

Hadad G. M., Abdel-Salam R. A., Abdel-Hameed E. A.

Acta Chromatographica

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2013

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Vol. 25, no. 3

431--450

EN

Two methods were presented for the simultaneous determination of two multicomponent mixtures containing pyridoxine hydrochloride (B6) and cyanocobalamine (B12) with benfotiamine (BN) [mix. 1], or thiamine nitrate (B1) and diclofenac sodium (DC) [mix. 2]. The first high-performance liquid chromatographic (HPLC) method depends on the use of a cyanopropyl column at a flow rate of 1.5 mL min-1 with a mobile phase consisting of acetonitrile-25 mM ammonium acetate, pH 3.7 (10:90, v/v), for mix. 1 or acetonitrile-25 mM ammonium acetate, pH 5.0 by gradient elution, changing the proportion of the system linearly with a time schedule program, for mix. 2. Quantitation was achieved by UV detection at 220 nm. The second method was based on HPTLC separation followed by densitometric measurement of the spots at 254 nm. The separation was achieved on HPTLC silica gel F254 plates using chloroform-ethanol-water-acetic acid (5:8:2:0.5, v/v/v/v) for mix. 1 and chloroform-ethanol-water-acetic acid (1.5:8:2:0.5, v/v/v/v) for mix. 2. The proposed methods were successfully applied for the analysis of pharmaceutical formulations containing the two multicomponent combinations.

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Development and validation of HPTLC-densitometric method for estimation of trigonelline in leaves of Abrus precatorius L. and its formulation

Kumbhalkar B. B, Rajopadhye A. A, Upadhye A. S

Acta Chromatographica

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2012

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Vol. 24, no. 3

511--518

EN

A new, simple and reproducible HPTLC-densitometric method has been established and validated for estimation of trigonelline in leaves of Abrus precatorius L. and its herbal formulation Chatak® using ICH guidelines. Accelerated solvent extraction (ASE) as an alternative to convention techniques was also explored for the rapid extraction. The methanol extracts of leaves, its formulation, and standard solution of trigonelline were applied on silica gel F254 HPTLC plates and developed in twin chamber using mobile phase toluene-ethyl acetate-formic acid-methanol (2:3:1.8:3.8, v/v/v/v). The plates were scanned at 268 nm (λmax of trigonelline) using Camag TLC scanner 3 with CATS 4 software. A linear relationship was obtained between response (peak area) and amount of trigonelline in the range 40–200 ng spot−1; the correlation coefficient was 0.9957. ASE method has higher extraction efficiency in less time as compared to Soxhlet extraction. The HPTLC-densitometric method showed good linearity, recovery and high precision of compound. It is useful to analyze trigonelline in A. precatorius leaves and routine quality control of its marketed formulation.

3

Development and validation of an HPTLC-densitometric method for the estimation of trigonelline in the leaves of Abrus precatorius L. and its formulation

Kumbhalkar B. B, Rajopadhye A. A, Upadhye A. S

Acta Chromatographica

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2012

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Vol. 24, no. 2

263--273

EN

A new, simple, and reproducible HPTLC-densitometric method has been established and validated for estimation of trigonelline in the leaves of Abrus precatorius L. and its herbal formulation Chatak® using ICH guidelines. Accelerated solvent extraction (ASE) as an alternative to conventional techniques was also explored for rapid extraction. The methanol extracts of leaves, its formulation, and standard solution of trigonelline were applied on silica gel F254 HPTLC plates and developed in a twin chamber using the mobile phase toluene-ethyl acetate-formic acid-methanol (2:3:1.8:3.8, υ/υ/υ/υ). The plates were scanned at 268 nm (λmax of trigonelline) using a Camag TLC scanner 3 with the CATS 4 software. A linear relationship was obtained between the response (peak area) and the amount of trigonelline in the range 40–200 ng per spot; the correlation coefficient was 0.9957. The ASE method gives higher extraction efficiency in less time compared to Soxhlet extraction. The HPTLC-densitometric method showed good linearity, recovery, and high precision. It is useful to analyze trigonelline in A. precatorius leaves and routine quality control of its marketed formulation.

4

Densitometric HPTLC analysis of cordifolioside A in Tinospora cordifolia and commercial formulations

Alam P., Ali M., Singh R., Shakeel F.

Acta Chromatographica

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2009

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Vol. 21, no. 4

683--692

EN

A densitometric HPTLC method for analysis of cordifolioside A both in 60% methanolic extract of Tinospora cordifolia and in a commercial formulation has been established and validated. Cordifolioside A was separated on aluminum-backed silica gel 60 F254 plates with chloroform-methanol 85:15 (%, v/v ) as mobile phase. A compact band was obtained for cordifolioside A at R F 0.52 ± 0.03. The limits of detection (LOD) and quantification (LOQ) were 20.12 and 60.36 ng per band, respectively. The highly precise and accurate method was used for analysis of cordifolioside A.