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Simultaneous determination of paeoniflorin and albiflorin in radix paeoniae rubra by HPLC—DAD—ELSD

Zhu M. X., Li S. N., You H. D., Han B., Hu Y. X., Li J., Liu Y. F.

Acta Chromatographica

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2017

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Vol. 29, no. 2

279--289

EN

High-performance liquid chromatography coupled with photodiode array detection and evaporative light scattering detection (HPLC—DAD—ELSD) was established to determine paeoniflorin and albiflorin simultaneously in Radix Paeoniae Rubra. The assay was performed on a Diamonsil C18 (4.6 mm × 250 mm, 5 μm) column by a gradient elution program with acetonitrile and aqueous formic acid (0.05% v/v) as mobile phase at a flow rate of 1.0 mL min−1. The detection wavelength of DAD was 230 nm, and the evaporator tube temperature of ELSD was set at 110 °C with the nebulizing gas flow rate of 3 L min−1. The temperature of column was kept at 30 °C. The linear ranges of paeoniflorin and albiflorin were within 0.050–1.510 mg mL−1 and 1.007–5.035 mg mL−1. The recoveries of paeoniflorin and albiflorin were 96.2–102.9% and 95.0–102.4%, respectively, while the relative standard deviation (RSD) of them was 0.2–2.5%. This method was quick, simple, accurate, and specific. It could be used for the quality control of Radix Paeoniae Rubra. The proposed approach was expected as a powerful tool for the quality control of Radix Paeoniae Rubra.

2

Chromatographic Fingerprint Analysis and Characterization of Constituents in Shenqi Fuzheng Injection by HPLC-DAD-ELSD and UFLC-DAD-Q-TOF Tandem Mass Spectrometry Techniques

Wang J., Liu M., Tong X., Peng W., Cao H., Su W.

Acta Chromatographica

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2015

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Vol. 27, no. 3

551--569

EN

Shenqi Fuzheng Injection (SFI) is a traditional Chinese medicine injection, widely used to enhance immune function of clinical cancer patients undergoing chemotherapy. In this study, a high-performance liquid chromatography-diode array detection-evaporative light scattering detection (HPLC-DAD-ELSD) method was established for quality control of SFI, which could simultaneously semiquantitatively reflect the constituents displayed in the chromatographic profile of SFI. The relative retention time and relative peak areas of the 21 common peaks related to the reference peak were calculated. The validity and advantage of this method were validated by systematically comparing chromatograms of 10 batches of SFI samples with the analytical methods of principal component analysis and angle cosine method recommended by the State Food and Drug Administration of China. Moreover, a total of 21 constituents of SFI were identified or tentatively characterized in the fingerprint via ultrafast liquid chromatography-diode array detection-quadrupole time-of-flight (UFLC-DAD-Q-TOF) tandem mass spectrometry technique on the basis of the retention time, ultraviolet spectra, fragmentation patterns, and reported literatures. All the results proved that the technique was useful in comprehensive quality evaluation of SFI and further study.

3

Quality evaluation of Yanghuo Sanqi tablet through a simultaneous determination of five major active flavonoids and three main saponins by HPLC-DAD-ELSD

Zhu F. X., Jia X. B., Cai Y., Chen Y.

Acta Chromatographica

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2013

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Vol. 25, no. 1

147--157

EN

Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography-diode array detector-evaporative light-scattering detector (HPLC-DAD-ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile-water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min-1. The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC-DAD-ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

4

Quality evaluation of Yanghuo Sanqi tablet through a simultaneous determination of five major active Flavonoids and three main saponins by HPLC-DAD-ELSD

Zhu F. X, Jia X. B, Cai Y., Chen Y.

Acta Chromatographica

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2012

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Vol. 24, no. 4

667--677

EN

Yanghuo Sanqi tablet (YST), combined prescription mainly derived from the leaves of Herba epimedii and the roots of Panax notoginseng, is a traditional Chinese medicine (TCM). Flavonoids (icarrin, epimedin A, epimedin B, epimedin C, and baohuoside I) and saponins (notoginsenoside R1, ginsenoside Rgl, and ginsenoside Rbl) are considered as the main bioactive compounds of YST. However, there is no report on quality control of TCMs by simultaneous determination of above-mentioned flavonoids and saponins so far. In this work, for the first time, a high-performance liquid chromatography-diode array detector-evaporative light scattering detector (HPLC-DAD-ELSD) method was developed to evaluate the quality of YST through a simultaneous determination of five major active flavonoids and three main saponins. Optimum separations were obtained with a Zorbax SB-C18 column by gradient elution with acetonitrile-water as the mobile phase. The drift tube temperature of ELSD was set at 105 °C, and the nebulizing gas flow rate was 2.5 L min-1. The fully validated method was successfully applied to quantify the eight bioactive components in three lot products. This simple, low-cost, and reliable HPLC-DAD-ELSD method provided a new basis for assessing the quality of traditional Chinese medicinal compound preparations (TCMCPs) consisting of many bioactive components.

5

Quantitative analysis of secondary metabolites in Centella asiatica grown under different habitats

Devkota A., Dall'Acqua S., Innocenti G., Jha P. K.

Chemia Analityczna

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2009

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Vol. 54, No. 1

69-75

EN

Centella asiatica (L) Urban is a very well known medicinal plant, widely used in the traditional medicine, pharmacy, and nutraceulical industry. Active constituents of this plant are triterpenes. Its main constituents, used nowadays as the markers for the assessment of the quality of drugs, are asiaticoside, madecassic acid, and asiatic acid. Centella asiatica occurs in Nepal and, if cultivated and harvested, might become a valuable resource. Thus, the major objective of this work was to determine the content of triterpenes: asiaticoside, madecassic acid and asiatic acid in the plants growing at different altitudes by HPLC-DAD-ELSD.

PL

Centella asiatica (L) Urban jest znaną rośliną leczniczą powszechnie używaną w tradycyjnej : medycynie, farmacji i w przemyśle odżywek. Aktywnymi składnikami tej rośliny są triter-peny, a ich głównymi składnikami, używanymi obecnie jako markery do oceny jakości leków, są: asiaticoside, madecassic acid i kwas azjatycki. Centella asiatica występuje w Nepalu i jej hodowla może stanowić wartościowe bogactwo naturalne. Celem pracy jest oznaczenie wymienionych składników triterpenu w roślinach rosnących na różnych wysokościach. Do oznaczania zastosowano wysokosprawną chromatografię cieczową z detektorami: evaporative light scattering i diode array detector.