Wyniki wyszukiwania - BazTech

1

Study of the effectiveness of the new method for the rapid PAHs analysis in soil

Włóka D., Placek A.

Acta Innovations

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2015

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no. 17

5--11

EN

The aim of this research was to compare the effectiveness of highly-selective chromatographic PAHs determination method with the new, simpler, non-selective spectrophotometric method, designed for the rapid monitoring of PAHs contamination level in soil. The conducted experiment includes the 2 series of PAHs content determinations, in 4 types of soils, with use of HPLC and spectrophotometric techniques. The obtained results, showed that the developed, PAHs content in soil determination method, can be used for quick and non-specific environmental monitoring of soil. However the effectiveness of this method is dependent on the content of organic matter in the analyzed soil samples.

2

HPLC analysis of Phyllanthus amarus samples stored in stability chambers under different conditions and study of the effect on quantification of the phytomarkers phyllanthin and hypophyllanthin

Srivastava P., Raut H. N., Puntambekar H. M, Desai A. C

Acta Chromatographica

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2015

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Vol. 27, no. 1

147--156

EN

Storage of medicinal plants may cause deterioration of the active principles with time, thus, reducing the efficacy of plants. Therefore, quantification of the active principle is essential before using the crude drug. Phyllanthus amarus (PA) contains lignans, namely, phyllanthin and hypophyllanthin, which shows anti-hepatotoxic activity. In this paper, we highlight the effect of storage conditions on the quantification of bioactive markers by high-performance liquid chromatography (HPLC) analysis in the crude plant material of PA. HPLC analysis of crude PA samples stored for certain period at long-term study (LS, 30 °C and 65% RH), accelerated study (AS, 40°C and 75% RH), and real-time study (RT) conditions was carried out using the LiChroCART Purospher® STAR RP-18 endcapped (250 × 4.6 mm, 5 μm) column along with a Purospher STAR RP 18e (4.0 × 4.0 mm, 5 μm) guard column using methanol:water (70:30) at a flow rate of 0.7 mL min−1 with ultraviolet (UV) detection at 220 nm. The HPLC study indicated that PA samples kept under LS condition are rich in lignan contents as compared to the samples stored under AS and RT study conditions. Therefore, PA should be used fresh to get maximum concentration of active lignans or it should be stored under LS conditions up to 6 months.

3

Chlorek chloroacetylu – metoda oznaczania

Kozieł E.

Podstawy i Metody Oceny Środowiska Pracy

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2006

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Nr 4 (50)

5--10

PL

Metoda polega na adsorpcji par chlorku chloroacetylu na żywicę XAD-7 z naniesioną 1-(2-pirydylo)piperazyną, desorpcji acetonitrylem otrzymanej pochodnej chlorku chloroacetylu i analizie otrzymanego roztworu metodą chromatografii cieczowej. Oznaczalność metody wynosi 0,02 mg/m3.

EN

The method is based on the chemisorption of chloroacetyl chloride on XAD-7 coated with 1-(2-pyridyl) piperazine, desorption of chloroacetyl chloride and 1-(2-pyridyl)piperazine derivative with acetonitryle and its determination in the obtained solution by high performance liquid chromatography. The determination limit of the method is 0,02 mg/m3.