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1 2009

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An RP-HPLC method for simultaneous analysis of, and interaction studies on, enalapril maleate and H 2 -receptor antagonists

Sultana N., Arayne M. S., Naveed S., Shamshad H

Acta Chromatographica

2009

Vol. 21, no. 4

547--558

A simple, sensitive, and rapid RP-HPLC method for analysis of enalapril in the presence of H 2 -receptor antagonists has been developed and validated. Enalapril maleate was separated from H 2 -receptor antagonists by use of a 250 mm × 4.6 mm, 5-µm particle, C 18 column with 86:14 ( υ / υ ) methanol-water, pH adjusted to 3.5, as mobile phase, at a flow rate of 1.5 mL min -1. UV detection was performed at 227 nm. The retention times of enalapril maleate, ranitidine, cimetidine, and famotidine were 3, 5, 7, and 7.5 min, respectively. The detection limit for enalapril was 10 ng mL -1 and the calibration plot was linear in the range 2.5–50 µg mL -1. In-vitro interaction of enalapril with the commonly administered H 2 -receptor antagonists cimetidine, ranitidine, and famotidine in simulated gastric juice at different pH and 37°C was also studied by use of this method. These studies clearly indicated that most of these H 2 -receptor antagonists bind to enalapril causing drastic changes in the availability of the drug. The HPLC method is accurate, selective, sensitive, and reproducible.