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Biochemical and cellular properties of Gluconacetobacter xylinus cultures exposed to different modes of rotating magnetic field

Fijałkowski K., Drozd R., Żywicka A., Junka A. F., Kordas M., Rakoczy R.

Polish Journal of Chemical Technology

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2017

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Vol. 19, nr 2

107--114

EN

The aim of the present study was to evaluate the impact of a rotating magnetic field (RMF) on cellular and biochemical properties of Gluconacetobacter xylinus during the process of cellulose synthesis by these bacteria. The application of the RMF during bacterial cellulose (BC) production intensified the biochemical processes in G. xylinus as compared to the RMF-unexposed cultures. Moreover, the RMF had a positive impact on the growth of cellulose-producing bacteria. Furthermore, the application of RMF did not increase the number of mutants unable to produce cellulose. In terms of BC production effi cacy, the most favorable properties were found in the setting where RMF generator was switched off for the fi rst 72 h of cultivation and switched on for the further 72 h. The results obtained can be used in subsequent studies concerning the optimization of BC production using different types of magnetic fields including RMF, especially.

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Effect of Gluconacetobacter xylinus cultivation conditions on the selected properties of bacterial cellulose

Fijałkowski K., Żywicka A., Drozd R., Kordas M., Rakoczy R.

Polish Journal of Chemical Technology

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2016

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Vol. 18, nr 4

117--123

EN

The aim of the study was to analyze the changes in the parameters of bacterial cultures and bacterial cellulose (BC) synthesized by four reference strains of Gluconacetobacter xylinus during 31-day cultivation in stationary conditions. The study showed that the most visible changes in the analyzed parameters of BC, regardless of the bacterial strain used for their synthesis, were observed in the first 10–14 days of the experiment. It was also revealed, that among parameters showing dependence associated with the particular bacterial strain were the rate and period of BC synthesis, the growth rate of bacteria anchored to the cellulose fibrils, the capacity to absorb water and the water release rate. The results presented in this work may be useful in the selection of optimum culturing conditions and period from the point of view of good efficiency of the cellulose synthesis process.

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Modification of bacterial cellulose to scaffold-like structures applied in process engineering

Kaźmierczak M., Olejnik T. P., Ogrodowczyk D., Kołodziejczyk M.

Biotechnology and Food Science

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2016

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Vol. 80, nr 2

91--96

EN

Scaffolds are three-dimensional structures which provides necessary support for different cells’ vital functions. Although they are widely produced from different materials, most of them are not biodegradable. Bacterial bionanocellulose (BNC) has this property and additionally, has similar features to natural, extracellular matrixes. Unfortunately, natural channels which are in BNC’s structure have not sufficient diameter to colonize them with, for example, mammalian cells. Some experiments for pores enlargement in cellulose structure have been conducted. Multiple frosting and defrosting of properly prepared BNC’s samples has produced some positive results. Application the mixture of sterile vegetable oil and ethanol at the cultured layers of bionanocellulose gave expected results – diameter of the channels and chambers is enough to colonize them with viable cells. The results of described experiments give hope that bacterial bionanocellulose, because of its transformation’s simplicity, could be an alternative material for bioplastics productions.