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Effect of the vapour phase on the separation of isoquinoline alkaloids by thin-layer chromatography

Petruczynik A., Śliwka K., Waksmundzka-Hajnos M.

Acta Chromatographica

2010

Vol. 22, no. 3

391--404

The effect of conditioning of the silica layer by mobile phase vapour, diethylamine vapour and its aqueous and methanolic solutions, and ammonia vapour on the retention of alkaloids eluted with multicomponent non-aqueous mobile phases was examined. Effect of conditioning time and vapour phase composition on system efficiency and peak symmetry was investigated, as also was the effect of vapour phase composition on separation selectivity. The most effective and selective systems were used for separation of the alkaloid fraction from Fumaria officinalis.