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1 Environment Protection Engineering

1 2011

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Dynamics of methanogenic archaeal communities based on the rrna analysis and their relation to methanogenic activity

Walczak M., Lalke-Porczyk E., Idzikowski W.

Environment Protection Engineering

2011

Vol. 37, nr 3

85-92

Changes in phylogenetic groups of methanogenic microorganisms during methane fermentation have been studied. Phylogenetic groups of methanogens were quantified and visualized by hybridization of oligonucleotide probes complementary to rRNA of the major phylogenetic groups. At the beginning of fermentation, Eubacteria are the main group and the count of Archaea constitutes only an insignificant percentage of the population of microorganisms. During the process of fermentation, a very significant increase in methanogenic microorganisms was recorded after 70 days of the process in progress. It is reflected in the quantity and the composition of released biogas.