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1 2005

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Adhesion and growth of vascular smooth muscle cells on protein assemblies for biomaterial coating

Filova E., Brynda E., Houska M., Riedel T., Bacakova L.

Inżynieria Biomateriałów

2005

R. 8, nr 47-53

9--12

We investigated adhesion and growth of vascular smooth muscle cells (VSMC) on proteins assemblies prepared by coating of thin fibrin net with ultrathin layer of collagen I, fibronectin or laminin. The assemblies were deposited on polystyrene (PS) non-modified with plasma discharge, i.e. a treatment commonly used for creation of surfaces suitable for cell cultivation. All samples with the fibrin assemblies improved initial attachment and spreading of VSMC as well as their subsequent growth in comparison with the pristine PS. The adhesion area of VSMC increased on fibrin coated with collagen I and fibronectin, while on laminin it remained similar as that on the pure fibrin. Thin 3D fibrin net markedly changed the morphology of VSMC from polygonal to star-like shape with protrusions. Cells all tested samples formed vinculin-containing focal adhesion plaques and α-actin cytoskeleton, an important marker of VSMC differentiation. The protein assemblies can be used for improvement of cell adhesion and growth on various artificial materials used in tissue engineering, e.g. for construction of bioartificial vascular prostheses.