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Synthesis of Deuterium, Tritium and 14-Carbon Labelled Isotopomers of L-DOPA

Kozłowska M., Kańska M.

Polish Journal of Chemistry

2007

Vol. 81, nr 10

1763-1763

The synthesis of five isotopomers of L-DOPAlabelled selectivelywith deuterium, tritium and 14C is reported. The isotopomers of L-tyrosine, the intermediate substrates for this synthesis, were obtained using chemical and enzymatic methods. Selectively labelled isotopomers of L-tyrosine were hydroxylated respectively to [3R-3H]-L, [5-2H]-L, [2 ,6 -2H2]-L, doubly labelled [5-2H/3H]-L, and [1-14C]-L-DOPA using activity of enzyme tyrosine (EC 1.14.18.1) isolated from mushrooms.