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Parts of the Polish oldest, illustrated Leopolita's Bible from XVI cent, are cleaned by the 532 nm Nd:YAG laser pulses and the results are analyzed by means of optical and scanning microscopy. Under mild irradiation conditions, i.e. for beam energy density from the range of 0.3-0.6 J/cm2, and pulse number not exceeding 15, the laser cleaning leads to positive results at locations where previously applied chemical treatment did not. This is confirmed by the microscopic observation and also by comparison of the respective fluorescence spectra obtained under laser excitation at 266 nm. It is shown, that for the case of laser application for cleaning of paper the careful selection of the applied wavelength, laser pulse energy and irradiation dose prevents the document and the paper substrate from unintended damage due to mechanical, thermal and photochemical effects.
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