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Występowanie Archaea w komunalnych osadach ściekowych i ich znaczenie w procesie biologicznego oczyszczania ścieków

Polus Michał, Anielak Anna M.

Przemysł Chemiczny

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2021

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T. 100, nr 10

977-980

PL

Badano różne osady ściekowe po biologicznym oczyszczaniu pod kątem występowania w nich mikroorganizmów utleniających amoniak: Archaea (AOA) i bakterii (AOB). Wyniki badań wykazały, że badane mikroorganizmy występują w ściekowym osadzie nadmiernym, osadzie po dezintegracji i osadzie przefermentowanym. Wyniki wskazują na możliwość izolacji i namnażania Archaea w warunkach oczyszczalni komunalnej.

EN

Samples of concd. activated sludge (both non-disintegrated and disintegrated) were subjected to semi-quant. detection of selected genes using the polymerase chain reaction method, carried out in a thermocycler. The sludges were tested for the presence of archaea and bacteria oxidizing NH₃. The tested microorganisms were present in excess sewage sludge, sludge after disintegration and fermented sludge. The results indicated the possibility of isolation and multiplication of archaea in the conditions of a municipal sewage treatment plant.

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The Effect of Bioaugmentation Strategy on the SBR Performance Treating Reject Water and Municipal Wastewater Under Various Temperature Conditions

Szaja Aleksandra, Szulżyk-Cieplak Joanna

Journal of Ecological Engineering

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2021

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Vol. 22, nr 7

46--56

EN

In this study, the effect of bioaugmentation on the sequencing batch reactor (SBR) performance while treating municipal wastewater and reject water under various temperature conditions was examined. Two lab-scale SBRs, each with the active volume of 8 L were used to perform this research. For bioaugmentation, a mixture of wildliving Bacteria and Archaea in a dose 0.25 mL was added to SBR A, while SBR B was a control one. Both reactors were fed with a mixture of wastewater and 13% v/v reject water. During the experiment, 5 phases with different temperature range were distinguished, each one lasted 14 d. The temperatures were investigated in 5°C increments, i.e. 20, 25, 30, 25 and 20°C. The obtained results indicated that in the bioaugmented reactor (SBR A), lower concentrations of NH4+–N, TSS, NO2-–N in effluent were observed as compared to control (SBR B). While for NH4+–N, regardless the temperature, the observed differences were statistically significant. Importantly, in both SBRs, the process was carried out in a stable way.

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Influence of Bioaugmentation Strategy of Activated Sludge on the Co-Treatment of Reject Water and Municipal Wastewater at a Decreasing Temperature

Szaja Aleksandra, Szulżyk-Cieplak Joanna

Journal of Ecological Engineering

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2020

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Vol. 21, nr 5

97--106

EN

In the present study, the influence of bioaugmentation strategy on the co-treatment of 13% v/v reject water and municipal wastewater at a decreasing temperature was evaluated. The experiment was performed in two identical laboratory sequencing batch reactors with the active volume of 8 L. Each one was operated using a 12-hour cycle at sludge retention time of 3 d. The SBR A was bioaugmented with a mixture of wild-living bacteria and Archaea in a dose 0.25 ml. In turn, the comparative reactor (SBR B) was non-bioaugmented, the Archaea product was replaced with an equal volume of dechlorinated tap water. The experiment was divided into 3 phases, each with a different temperature range (20, 15 and 10°C). The temperature reduction did not adversely affect the process performance in the bioaugmented and non-bioaugmented system. Significant removal efficiencies were achieved in both SBRs. The major differences were observed for the COD content in the bioaugmented SBR at a temperature of 10°C. In this case, statistically lower concentrations in the effluent were observed in the bioaugmented system than in the non-bioaugmented one. Additionally, at a temperature of 10°C, an improved process performance was observed in the Archaea presence, but the differences were of no statistical significance.

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The use of Archaea in the bioaugmentation of activated sludge as a method for the biological removal of nitrogen compounds

Polus M., Anielak A. M.

Technical Transactions

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2017

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Vol. 5(114)

83--95

EN

This paper examines the effect of Archaea on wastewater treatment in sequencing biological reactors (SBR). The research was carried out in two SBR reactors: a reactor with activated sludge bioaugmented with Archaea (microorganisms which constitute a third domain besides Bacteria and Eukaryotes); a reactor with conventional activated sludge was used as a control. Archaea were incubated in laboratory conditions as recommended by Archaea Solutions Inc. The research revealed that the time period required for the acclimation of the activated sludge in the presence of Archaea was twice as long as in the case of regular nitrifying activated sludge. The observed nitrogen and phosphorous removal from wastewater was achieved to a higher extent in sludge with Archaea and the sludge itself settled faster. The required concentration of oxygen in the reactor with Archaea was lower than in the classic set-up – this resulted in lowering the operating costs of the treatment plant. Furthermore, the denitrification process was significantly shorter and did not require nitrate nitrogen (V).

PL

W pracy przebadano wpływ archeanów na proces oczyszczania ścieków w cyklicznych reaktorach biologicznych. Badania przeprowadzono w dwóch reaktorach typu SBR, z których jeden był poziomem odniesienia (oczyszczanie w warunkach klasycznych), a w drugim oczyszczano ścieki osadem czynnym poddanym bioaugmentacji archeanami, mikroorganizmami stanowiącymi trzecią domenę obok bakterii i eukariontów. Archeany były inkubowane w warunkach laboratoryjnych wg metody zalecanej przez ArchaeaSolutions, Inc. Badania wykazały, że adaptacja osadu czynnego do pracy w obecności archeanów wymaga dwa razy dłuższego czasu, niż zwykły osad czynny, zdolny do efektywnej nitryfikacji. Efektywność usuwania związków azotu i fosforu ze ścieków w obecności archeanów jest większa, a osad czynny szybciej sedymentuje. Wymagane stężenie tlenu w reaktorze z archeanami jest mniejsze niż w układzie klasycznym, co zmniejsza koszty eksploatacji oczyszczalni. Proces denitryfikacji jest znacznie skrócony i nie wymaga obecności azotanów(V).