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Polyphasic characterization of the genus Anabaena from the Gulf of Gdańsk (Southern Baltic Sea). Application of DGGE in taxonomic studies on environmental samples

Siedlewicz G., Forycka K., Surosz W.

Oceanological and Hydrobiological Studies

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2012

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Vol. 41, No. 3

64-78

EN

The aim of this paper was a taxonomic verification of cyanobacteria of the genus Anabaena that occur in the Gulf of Gdańsk. Classical taxonomic methods were combined with modern molecular taxonomic methods (polyphasic approach). Analysis of the species diversity of cyanobacteria from the genus Anabaena was based on the microscopic analysis and on the analysis of 16S rRNA and ITS region. Comparison of the obtained results with sequences in GenBank showed 97.8-100% similarity for 16S rRNA and 98.8-100% similarity in the case of the ITS fragment. Similarity of the 16S rRNA and ITS sequence of 98.5% seems to be sufficient to determine the species.

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Blooms of toxin-producing Cyanobacteria - a real threat in small dam reservoirs at the beginning of their operation

Pawlik-Skowrońska B., Toporowska M.

Oceanological and Hydrobiological Studies

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2011

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Vol. 40, No.4

30-37

EN

Large and harmful cyanobacterial blooms appeared in two newly-built artificial reservoirs shortly after being filled with water. Taxonomic composition of cyanobacterial communities was highly variable in both water bodies and fast species replacement was observed. In the first year of the operation of the smaller Konstantynów Reservoir, the mass development of Anabaena flos-aquae and Planktolyngbya limnetica (48.7 and 53.6% of the cyanobacterial abundance) occurred in summer, while in autumn the dominance of Planktothrix agardhii (99.9%, 14.95 × 106 ind. dm-3 ) was noted. The surface scum developed in summer consisted of An. flos-aquae that contained high amounts of anatoxin-a (1412.4 μg AN-a dm-3 of scum) and smaller amounts of microcystins (10 μg eq. MC-LR dm-3 of scum). In the larger Kraśnik Reservoir, Aphanizomenon flos-aquae occurred in high abundance in spring and summer, however, it was replaced created thick surface scum. Simultaneously, a hazardous increase in the total concentration of microcystins (from 13.6 to 788.5 μg eq. MC-LR dm-3 of water with scum) and anatoxin-a (from 0.03 to 43.6 μg dm-3) was observed.

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Relationship between Cyanobacterial bloom composition and anatoxin-a and microcystin occurrence in the eutrophic dam reservoir (SE Poland)

Pawlik-Skowrońska B., Skowroński T., Pirszel J., Adamczyk A.

Polish Journal of Ecology

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2004

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Vol. 52, nr 4

479-490

EN

Long-lasting cyanobacterial blooms were observed over summer periods 2001-2003 in the highly eutrophic dam reservoir (Zemborzycki Reservoir near Lublin, SE Poland) loaded with nitrogen and phosphorus by the river Bystrzyca. Chloriphyll a content and water transparence reached in summer periods 2002-2003 average values 115-129 [mi]g l^-1 and 0.5 m, respectively. The blooms consisted of eight taxa of Cyanobacteria occurring in different proportions: Aphanizomenon flos-aquae, Anabaena circinalis, Anabaena spiroides, Anabaena flos-aquae, Planktothrix agardhii, Microcystis aeruginosa, Microcystis viridis, Microcystis wesenbergii. At decreased average water temperature and increased concentration of dissolved ammonium-nitrogen in summer 2003, compared with two earlier years, a replacement of dominant Aph. flos-aquae to P. agardhii was observed. The total abundance of potential microcystin producers was positively correlated with the content of these hepatotoxins in the reservoir water. First time in Poland, mass occurrence of Anabaena strains producing anatoxin-a was observed. Anatoxin-a concentration in the reservoir water was positively correlated with total abundance of 3 taxa of Anabaena. It was no such correlation with Aph. flos-aquae.