Wyniki wyszukiwania - BazTech

1

Amplified fragment length polymorphism (AFLP)-based differentiation of selected Chara species

Urbaniak J., Combik M.

Oceanological and Hydrobiological Studies

|

2017

|

Vol. 46, No. 1

74--84

EN

Charophytes are a group of green algae that grow in various types of water ecosystems and are characterized by a high degree of plasticity and morphological variation. To analyze the genetic diversity and taxonomic rank of several species from the genus Chara, the fingerprinting technique of Amplified Fragment Length Polymorphism (AFLP) was applied. We studied species that belong to sect. Grovesia (C. tenuispina, C. globularis, C. virgata, C. aspera and C. strigosa) and two species from the sect. Hartmania (C. intermedia and C. hispida). The individuals were collected in the field in north-eastern, central and eastern Poland. The species were identified based on morphological features and then analyzed using the AFLP fingerprinting method. UPGMA clustering and PCA analysis as well as morphological analysis revealed a clear separation of C. tenuispina and C. globularis, which formed separate clusters supported by high bootstrap values. Therefore, these species were distinguish as separate taxa, rather than varieties of C. globularis. Similarly, C. virgata also formed a separate cluster, thereby confirming that this taxon is a separate species, rather than a variety of C. globularis. The AFLP analysis did not show any differentiation between C. aspera and C. strigosa. The presented results do not fully support the taxonomic interpretation for the existence of several polymorphic species with numerous variations and forms, however, in some examples, the distinctive nature of the reproduction system may be used as a distinguishing feature of the taxa.

2

Influence of neighboring tree species on AFLP variants of endangered Picea chihuahuana Martínez populations on the Sierra Madre Occidental, North-wastern Mexico

Quiñones-Pérez C. Z., Sáenz-Romero C., Wehenkel C.

Polish Journal of Ecology

|

2014

|

Vol. 62, nr 1

55--65

EN

Plant-plant interactions, which are defined as the effect of one plant on another of the same or different species, are inherently local in nature. Plants interact in both positive and negative ways, which are governed by the same underlying ecological dynamic processes. The Mexican endemic Picea chihuahuana Martínez is a largely untouched tree species and it is therefore an excellent model for estimating genetic potential of plant-plant interactions under natural conditions. Because of the scarce research examining plant-plant interactions at the genetic level, we tested the hypothesis that specific genetic variants of P. chihuahuana are associated with (and likely selected by) the presence of particular neighbouring tree species. To explore this question, we applied AFLP genotyping to five P. chihuahuana populations in the State of Durango, North-western Mexico. Under the assumption that the variables genetic variant of P. chihuahuana and nearest-neighbour tree species are directly related to each other, the results show a statistically significant association between such genetic variants – at one AFLP locus in one Picea population – and two nearest-neighbour conifer species (P. chihuahuana Martínez and Pinus arizonica Engelm.). The findings indicate that interactions between genetic variants and species diversity may be crucial in shaping plant communities. However, further studies are required for a better understanding of the possible roles that such associations between genetic variants might play.

3

Genetic diversity of populations of Brachypodium pinnatum (L.) P. Beauv. : expansive grass in a fragmented landscape

Bąba W., Kurowska M., Kompała-Bąba A., Wilczek A., Długosz J., Szarejko I.

Polish Journal of Ecology

|

2012

|

Vol. 60, nr 1

31-40

EN

Brachypodium pinnatum belongs to native grasses which could dramatically decrease the biodiversity of calcareous grasslands, therefore, an examination of the mechanisms of its expansion is of prime importance for their conservation. We studied the genetic structure of 12 subpopulations of the expansive grass B. pinnatum in a heterogeneous landscape with AFLP markers, aiming at determination whether spatial isolation influences the genetic diversity and the population genetic structure of this species. A high level of overall (79%) and within population polymorphic loci (38.44%) were found and 220 different genotypes were distinguished among the 252 samples analysed. No significant population structure nor isolation-bydistance were found, despite their long-time fragmentation history. This confirms that isolation of calcareous grasslands in a landscape cannot prevent the expansion of B. pinnatum grasses because of the high generative dispersal ability. Subsequent quick and extensive clonal growth of this species enables the successful establishment. In most cases mowing or grazing is sufficient to keep this species at a low density within ancient grasslands and to prevent seed and pollen dispersal, but not for restoration of species-rich calcareous grasslands.

4

Genetic variability of small isolated populations of Cicerbita alpina (L.) Wallr. (Asteraceae) in the Beskid Mały Mts (southern Poland)

Stachurska-Swakoń A., Cieślak E., Ronikier M.

Polish Journal of Ecology

|

2011

|

Vol. 59, nr 2

279-288

EN

The populations of Cicerbita alpina in the Beskid Mały Mts. (Western Carpathians, Poland) are the northernmost, spatially isolated localities of this subalpine tall-herb species in the Carpathians. The genetic structure of these populations was studied using the AFLP method. The analysis also included the populations of the larger, more population-abundant parts of the distribution range to the north (Scandinavia) and to the south (the Tatra Mts., Western Carpathians). The genetic similarity of the Beskid Mały populations with those from the Tatra Mts. and Scandinavia was relatively low and the populations formed geographically distinct genetic groups. The populations from the Beskid Mały Mts. were characterised by lower genetic variation, as well as the lowest degree of genetic differentiation (Nei and Shannon's coefficients), compared with those from the Tatra Mts. and Scandinavia. Our results indicate a relatively long period of isolation of the Beskid Mały Mts. populations; there is no evidence for recent dispersal or extant gene flow with populations from other regions. The differences among the populations also testify to fixation of genes in isolated areas, probably traced back to the founder individuals.

5

Genetic variability in the Antarctic hairgrass Deschampsia antarctica Desv. From maritime Antarctic and Subantarctic sites

Chwedorzewska K., Bednarek P.

Polish Journal of Ecology

|

2008

|

Vol. 56, nr 2

209-216

EN

The level of polymorphism, genetic variability and relatedness of the Antarctic hairgrass Deschampsia antarctica Desv. populations from South Shetlands Is. (King George I., Penguin I., Livingstone I.), from vicinity of Antarctic Peninsula (Galindez I., Uruguay I.), and Falklands Is. (Jason Is., Sealion I.) were studied using the AFLP approach. Six EcoRI/MseI type selective primer pairs combinations were used for AFLP profiling and amplified scoreable DNA fragments; than AMOVA and PCA were performed. The level of molecular variability among all individuals from all the analysed populations was low and reach only 5.4 % irrespective of the distance between the localities and it was not significant at a broader geographical scale, even among the three groups of populations (South Shetlands Is., vicinity of Antarctic Peninsula, and Falklands Is.). PCA analysis shows that all populations formed uniform groups according to their locations.