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3D morphological reconstruction of the red blood cell based on two phase images

Wang Y, Li Z, Ji Y, Xu Y, Bu M

Optica Applicata

2015

Vol. 45, nr 2

173--182

As an important component of blood cells, the red blood cell plays a vital role in many diseases such as malaria and so on. Although quantitative phase imaging techniques can be used for homogeneous cellular thickness distribution to obtain ideal results, they cannot achieve 3D morphological distribution. In this paper, a new method is presented to get a 3D morphology image of red blood cell. With this method, only two cellular quantitative phase images obtained from two orthogonal directions are needed as original information. By using the grid method, the sample is divided into many small phase cubes, and then we take a layer’s cubes into calculation so that the 3D problem could be transformed into a 2D problem to elaborate. Then it can be applied to the tomographic imaging combined with the maximum entropy method according to the two orthogonal phase images. This method has been proved by a simulation of red blood cell. The results show that cellular morphological distribution can be achieved in detail very well just based on only two orthogonal phase images.