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Investigation of the toxicity of the ionic liquid 1-butyl-3-methylimidazolium chloride to Saccharomyces cerevisiae AY93161 for lignocellulosic ethanol production

Zhu Shengdong, Yu Pei, Lei Mingke, Tong Yanjie, Zheng Lu, Zhang Rui, Ji Jun, Chen Qiming, Wu Yuanxin

Polish Journal of Chemical Technology

2013

Vol. 15, nr 2

94–98

Ionic liquid (IL) pretreatment of lignocellulosic materials has provided a new technical tool to improve lignocellulosic ethanol production. To evaluate the influence of the residual IL in the fermentable sugars from enzymatic hydrolysis of IL pretreatment of lignocellulosic materials on the subsequent ethanol fermentation, the toxicity of the IL 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) to Saccharomyces cerevisiae AY93161 was investigated. Firstly, the morphological structure, budding and metabolic activity of Saccharomyces cerevisiae AY93161 at different [BMIM]Cl concentrations were observed under an optical microscope. The results show that its single cell morphology remained unchanged at all [BMIM]Cl concentrations, but its reproduction rate by budding and its metabolic activity decreased with the [BMIM]Cl concentration increasing. The half effective concentration (EC50) and the half inhibition concentration (IC50) of [BMIM]Cl to Saccharomyces cerevisiae AY93161 were then measured using solid and liquid suspension culture and their value were 0.53 and 0.39 g.L-1 respectively. Finally, the influence of [BMIM]Cl on ethanol production was investigated. The results indicate that the [BMIM]Cl inhibited the growth and ethanol production of Saccharomyces cerevisiae AY93161. This toxicity study provides useful basic data for further development in lignocellulosic ethanol production by using IL technology and it also enriches the IL toxicity data.

Conductivity of 1-Butyl-3-methyl-imidazolium Chloride Solutions in Alcohols

Domańska U., Bogel-Łukasik E.

Polish Journal of Chemistry

2006

Vol. 80, nr 1

189-203

This work presents the ionic conductivity of 1-butyl-3-methylimidazolium chloride [bmim][Cl]'s solutions in alcohols (ethanol, 1-butanol, 1-hexanol, 1-octanol, 1-decanol, 2-butanol, 2-methyl-2-propanol) and that of pure alcohols measured in wide range of temperature and concentration. The values of limiting conductance were determined by the Onsager limiting law. The molar conductivities of these systems were presented as a function of temperature and concentration. The mathematical description of ionic conductivities data by the Vogel-Tammen-Fulcher equation was made. It was shown that the conductivity of [bmim][Cl]'s solutions in the relatively low dielectric constant media is affected by the length of an alcohol carbon chain.