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Assessment of seasonal variation of the bioactive oleuropein in Olea europaea L. leaves cultivated in Saudi Arabia

Alqarni Mohammed Hamed, Salkini Mohamad Ayman, Alam Prawez, Alanazi Mazen Talal, Abdel-Kader Maged Saad, El Sohafy Samah M.

Acta Chromatographica

2022

Vol. 34, no. 3

297--303

Plants secondary metabolites undergoes qualitative and quantitative variation due to environment al and growth factors. It is a crucial factor to select the proper time for collection of medicinal plants to assure maximum content of active components reflected as maximum efficacy. Olive leaves (Oleaeuropaea L.) are known traditionally for their antidiabetic effect. The secoiridoid glycoside oleur-opein is the main active component of Olive leaves responsible for the biological activity. The current study was conducted to monitor the seasonal variation of oleuropein in Olives leaves collected from the same location. To achieve this goal a validated HPLC method following the ICH guidelines was established. Separation was conducted using RP18 column and a mobile chase consisted of ultrapure water containing 20% acetonitrile and 1% acetic acid. Detection was performed at 254 nm with 1 mL/min flow rate. The method was simple, linear, accurate, precise, specific and robust. The analyses revealed considerable variations in the level of oleuropein throughout the year. This variation cannot be explained by temperature variation during the year. Two points of high levels of oleuropein were detected prior to flowering stage and ripening of the fruits. The levels of growth regulators most likely is responsible for the increased production of oleuropein. It is recommended that leaves intended for medicinal use to be collected during the fruiting stage prior to fruit ripening.

Separation and quantification of nine bioactive compounds in traditional Unani formulations by High Performance Liquid Chromatography–Photodiode Array Detector

Kamal Y. T., Ahmad Sayeed, Mahadevan Nanjaian, Alam Prawez, Salam Shahana, Asiri Yahya, Muhsinah Abdullatif Bin, Alsayari Abdulrhman

Acta Chromatographica

2021

Vol. 33, no. 1

3--10

A new High Performance Liquid Chromatography–Photodiode Array Detector (HPLC–PDA) method has been developed for the chromatographic separation and simultaneous quantitative determination of nine bioactive compounds, i.e. four phenolic (gallic acid, ellagic acid, chebulinic acid, and tannic acid), two flavanoids (rutin and quercetin), two anthraquinones (sennoside A and B) and one oxygenated hydrocarbon (vitamin C) in a well-known Unani polyherbal formulation namely Itrifal’s. Separation was accomplished on a C18 LiChrospher 100 column (5 mm, 250 3 4.6 mm) with a gradient elution and recorded at 254 nm. The results demonstrated that the proposed method is reproducible, accurate, economic, and suitable for the quality control of traditional polyherbal Unani formulations containing complex compounds with different structures such as Itrifals.