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1 2010

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Image Processing System for Localising Macromolecules in Cryo-Electron Tomography

Gedda M., Öfverstedt L. G., Skoglund U., Svensson S.

Machine Graphics and Vision

2010

Vol. 19, No. 2

159-184

A major challenge in today's molecular biology research is to understand the interaction between proteins at the molecular level. Cryo-electron tomography (ET) has come to play an important role in facilitating objective qualitative experiments on protein structures and their interaction. Various protein conformation structures can be qualitatively analysed as complete galleries of proteins are captured by ET. To facilitate fast and objective macromolecular structure analysis procedures, image processing has become a crucial tool. This paper presents an image processing system for localising individual proteins from in vitro samples imaged by ET. We have evaluated the system using simulated data as well as experimental data.