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Recycling preparative high performance liquid chromatography for the separation of curcumin from curcuminoids in curcuma longa L.

Mollayi S., Tamhidi S., Hashempour H., Ghassempour A.

Acta Chromatographica

2015

Vol. 27, no. 2

387--398

Curcumin is the main bioactive constituent of turmeric rhizome. A wide range of pharmacological activities of curcumin has been reported. There has been growing interest in the development of preparative purification of curcumin. The objective of this study is the isolation of curcumin from other components by using recycling preparative HPLC method from curcuma longa L. The extraction was achieved by using ultrasonic system with three different solvents, including acetone, dichloromethane, and ethanol. After optimization of extraction process, preparative HPLC system was first applied for separation of curcumin. Because of poor resolution of preparative HPLC, recycled preparative HPLC was applied for separation of curcumin with higher purity up to five cycles. Recycled preparative HPLC resulted in improvement in separation factors which led to the increase of curcumin purity up to 99.5%. This method is simple, highly efficient, environmentally friendly, and has been demonstrated to be effective for preparation and purification of curcumin from curcuma longa L.

Multidimensional liquid chromatography for separation of cyclotides in Viola ignobilis

Ghassempour A, Ghahramanzamaneh M, Hashempour H, Kargosha K

Acta Chromatographica

2011

Vol. 23, no. 4

641--651

In this study, different columns, including C18, C8, and CN, were investigated for the analysis of cyclotides in Viola ignobilis. Because of the complexity of the cyclotide matrix in V. ignobilis and the presence of their different isomers, suitable separations could not be obtained by high-performance liquid chromatography (HPLC). Also, a miniaturized column with a powerful determination technique, such as nanoliquid chromatography-Fourier transform mass spectroscopy (nano-LC-FTMS), could not succeed in the identification of all the cyclotides. Thus, a multidimensional liquid chromatography (MDLC), in the heart-cutting and mixed-bed modes, has been developed to separate the cyclotides. In the heart-cutting MDLC, the C8-C18 and C8-CN conditions were investigated and a good resolution could be obtained for the isolation of a few specific peaks. In the mixed-bed mode, a C8-CN condition was investigated and good separation was obtained by a gradient condition. The results of these methods are compared.