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1 2009

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Evaluation of a CGC-FID method for qualitative and quantitative analysis of azole antifungal drugs

Ekiert R. J., Krzek J., Czekaj J. S., Hubicka U.

Acta Chromatographica

2009

Vol. 21, no. 2

273--282

This paper presents a new method for identification and quantitative analysis of six azole antifungal drugs — bifonazole, clotrimazole, econazole, fluconazole, ketoconazole, and miconazole — by capillary gas chromatography (CGC) combined with flame ionization detection (FID). The chromatographic separation conditions were established and the method was validated for precision (RSD = 1.49–3.55%), recovery (98.6–101.2%), and linearity within the range under investigation (∼1.0–33.3 ng). The results obtained show the newly developed procedure is suitable for qualitative and quantitative pharmaceutical analysis of the six azoles.