The purpose of the work was to conduct biotesting for the presence of antibiotics in several lakes of the Akmola region, located in the zone of possible ingress of antimicrobial drugs into them. The adopted research method was the modified disk diffusion method, employed to determine the microorganisms’ sensitivity to the used antibiotics. As a result of the study, sensitivity to antibiotics remained in the main control lakes. The antibiotics that have not been found to be resistant to microorganisms in lakes included: enronite, furagin, cefuroxime, cefoperazone, and amikacin. The most common resistance of microorganisms 90–100% was in such antibiotics as benzylpenicillin, kanamycin, streptomycin, tylosin, trimethoprim/sulfamethoxazole, metronidazole, amikacin, and spectinomycin.
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