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Aqueous biological graphene based formulations for ink-jet printing

Dybowska-Sarapuk Ł., Rumiński S., Wróblewski G., Słoma M., Młożniak A., Kalaszczyńska I., Lewandowska-Szumieł M., Jakubowska M.

Polish Journal of Chemical Technology

2016

Vol. 18, nr 2

46--52

The aim of the study was to produce heterophasic graphene nanoplatelets based formulation designed for ink-jet printing and biomedical applications. The compositions should meet two conditions: should be cytocompatible and have the rheological properties that allow to apply it with ink-jet printing technique. In view of the above conditions, the selection of suspensions components, such as binder, solvent and surfactants was performed. In the first stage of the research the homogeneity of the dispersion of nanoplatelets and their sedimentation behaviour in diverse solutions were tested. Subsequently, the cytotoxicity of each ink on human mesenchymal stem cells was examined using the Alamar Blue Test. At the same time the rheology of the resulting suspensions was tested. As a result of these tests the best ink composition was elaborated: water, polyethylene glycol, graphene nanoplatelets and the surfactant from DuPont company.

Effect of polylactide modification with β-TCP and lecithin on the properties of the material as a substrate for osteoblasts

Olkowski R., Stefanek A., Kaszczewski P., Ciach T., Lewandowska-Szumieł M., Kalaszczyńska I.

Engineering of Biomaterials

2015

Vol. 18, no. 131

8--11

Polylactide (PLLA) containing β-TCP is biodegradable composite and an attractive biomaterial for bone tissue engineering, however, hydrophobicity of PLLA based composites is major limitation for their use as scaffolds for cell culture. In our study lecithin was used to improve hydrophilicity and cytocompatibility of PLLA/ β-TCP composite. Thin films of PLLA, PLLA/ β-TCP and PLLA/β-TCP/lecithin were manufactured by solvent-casting technique. Comparative analysis of all types of films was performed. Addition of β-TCP did not change hydrophilicity of PLLA. The hydrophilicity of PLLA/β-TCP/lecithin increased in comparison to PLLA and PLLA/β-TCP. Degradation of PLLA/β-TCP composite surpassed the degradation of PLLA while addition of lecithin diminished the degradation of composite. The cytocompatibility of composites were studied in 7 day long in vitro assay. Human bone derived cells were seeded on all tested surfaces. Cell viability was estimated by Live/Dead fluorescent staining and Alamar Blue test. Surprisingly, although lecithin addition improved hydrophilicity of the PLLA-based composite, adhesion and proliferation of human bone derived cells were markedly hampered on PLLA/β-TCP/lecithin in comparison to PLLA and PLLA/β-TCP. Despite positive effect we found of lecithin addition on hydrophilicity and stability of PLLA-based composite, its effect on cell attachment and proliferation is negative. Hence, incorporation of lecithin did not improve properties of PLLA/β-TCP/lecithin composite intended for bone tissue regeneration.