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In - house preparation and evaluation of Tc-99m-HIG kit for detection of infection in AIDS patients

Bakir M. A., Mansour R., Mohammad A., Habil K., Yassine T.

Nukleonika

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2012

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Vol. 57, No. 1

117-124

EN

99mTc-HIG can be used for early detection of inflammation and infection foci especially in immunodeficiency patients. The present study involves in-house preparation and evaluation of human immunoglobulin-hydrazinonicotinic acid (HIG-HYNIC) kit to be labeled with 99mTc. In this indirect labeling method, the structure and biological activity of the protein can be maintained. The kit consists of two vials A and B, the vial A contains 2 mg of HIG-HYNIC, and the vial B contains 10 mg of tricine and 1 mg of stannous chloride dihydrate (SnCl2.2H2O). The results revealed that the lyophilized kits were stable for at least 10 months, and showed a high quality from the points of physical, chemical, radiochemical and biological purities, and its validity for human injection. The labeling yield of HIG-HYNIC compound exceeded 98%. The application of the prepared kit was experimentally illustrated in animal models with artificially induced inflammatory lesions after E. coli injection into rat leg muscle. The biodistribution and imaging studies in rats have shown good localization in infected muscle (T) in comparison to the normal muscle (M) 4 and 24 h after injection. Ratios of 17 and 18 were achieved 4 and 24 h post injection, respectively. The results of biodistribution of 99mTc-HIG in human and initial clinical evaluation show that this agent is a good radiopharmaceutical for infection imaging.

2

Production of 166Ho and 153Sm using hot atom reactions in neutron irradiated tris(cyclopentadienyl) compounds

Nassan L., Achkar B., Yassine T.

Nukleonika

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2011

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Vol. 56, No. 4

263-267

EN

The behavior of a recoiled 166Ho and 153Sm resulting from the reactions 165Ho(n,gamma)166Ho and 152Sm(n,gamma)153Sm in the organometallic compounds tris(cyclopentadienyl)holmium (C5H5)3Ho(III), and tris(cyclopentadienyl)samarium (C5H5)3Sm(III) were investigated. Several chemical separation methods were developed for each compound to separate the recoil products from the irradiated compound. Retention value of 0.2 �} 0.1% was obtained for (C5H5)3Ho(III) using sublimation. Another value of retention of 0.4 plus or minus 0.1% resulted when column chromatography was used. Paper chromatography (using Whatman Chr. 1 and Whatman Chr. 4) gave different retention values of about 0.11 plus or minus 0.05%, and 0.4 plus or minus 0.1%, respectively. Similar behavior of 153Sm in irradiated (C5H5)3Sm(III) was found when paper chromatography was used for separation. The different retention values given by different methods of separation were discussed. An important improvement of the specific activity was achieved as compared with other published results in this field. The obtained results pointed out that tris(cyclopentadienyl)holmium and samarium could be used to produce high specific activity of 166Ho and 153Sm in the Syrian low flux miniature nuclear source reactor (MNSR), where the averaged achieved enrichment factor for holmium compound was found to be greater than 1.6 x 107.

3

Post-recoil thermal annealing study of 177Lu, 169Yb, 175Yb, 166Ho and 153Sm in different organometallic compounds

Nassan L., Achkar B., Yassine T.

Nukleonika

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2011

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Vol. 56, No. 2

185-190

EN

Post-recoil thermal annealing study at different temperatures for different intervals of time of the recoiled isotopes 169Yb, 175Yb, 177Lu, 166Ho, and 153Sm resulting from the nuclear reactions 168Yb(n,gamma)169Yb, 174Yb(n,gamma)175Yb and 176Yb(n,gamma)177Yb → 177Lu, 165Ho(n,gamma)166Ho, 152Sm(n,gamma)153Sm, respectively in different organometallic compounds Yb(OCC(CH3)3CHCOC(CH3)3)3, Ho(OCC(CH3)3CHCOC(CH3)3)3, (C5H5)3Ho, and (C5H5)3Sm were carried out. The thermal annealing behaviors of 177Lu, 169Yb, 175Yb, 166Ho, and 153Sm were found to be different in the compounds under investigation. The differences in the patterns of the obtained isothermal curves were discussed. Depending on the post- -recoil thermal annealing manner the reactions taking place during thermal annealing were suggested.