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Densitometric HPTLC and HPLC analysis of phenolic acids from Aquilegia vulgaris

Szaufer-Hajdrych M., Bylka W., Matławska I., Wójciak-Kosior M., Matysik G., Jodynis-Liebert J.

Acta Chromatographica

2008

Vol. 20, no. 4

685-695

Densitometric HPTLC and HPLC have been used for quantification of p -coumaric and protocatechuic acids in an ethereal fraction from a methanolic extract of Aquilegia vulgaris L. HPLC analysis was performed on an RP-18 column with methanol-water-formic acid 25:75:0.5 ( υ/υ ) as mobile phase. Thin layer chromatography was performed on Si60 F 254 HPTLC plates with mixtures of heptane, dichloromethane, diisopropyl ether, formic acid, and water as mobile phases. Satisfactory separation of the phenolic acids was achieved by use of the multiple gradient development technique. The quantities of p -coumaric and protocatechuic acids determined by HPLC were 0.374 and 2.283 mg g -1 dry plant material, respectively; HPTLC results were somewhat higher - 0.396 and 2.584 mg g -1 , respectively. The precision of both methods, expressed as relative standard deviation, was satisfactory. The methods are useful for quality control of Aquilegia vulgaris extracts.

Determination of oleuropein in herbal preparation and Olea europaea L. extracts by HPLC

Szymański M., Witkowska-Banaszczak E., Matławska I., Szymański A.

Ars Separatoria Acta

2012/2013

No. 9/10

55--63

Oleuropein is a secoiridoide monoterpene. It is found primarily in all parts of the Olea europaea L. tree, mostly in its unripe fruit and leaves. It has a number of beneficial effects on the human body, being readily absorbed from the gastrointestinal tract due to its hydrophilic properties. The paper presents a simple, rapid and precise method for the assay of oleuropein in herbal formulations and the European Olive leaf extract. Chromatographic separation was performed using a C-18 column with an acetonitrile/water 20/80% vol. mixture as the mobile phase. The method has a wide range of linearity between 0.05 and 1.5 mg/mL, very good repeatability of retention time, and accuracies between 95.8 and 103.3%. Its limit of detection is 0.04 mg/mL. The developed method was tested on two herbal formulations which are commercially available in Poland.