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Vascular endothelial cells in cultures on metal/C:H composite films

Bacakova L., Noskova L., Koshelyev H., Biederman H.

Inżynieria Biomateriałów

2004

R. 7, nr 37

18-20

Adhesion, proliferation and maturation of vascular endothelial cells in cultures derived from the bovine pulmonary artery (line CPAE) were studied on Ti/C:H or Ag/C:H films prepared on glass using dc magnetron plasma deposition. The concentration of Ti or AG in the films ranged from 0% to 20%. On Ag/C:H layers, increasing concentration of silver markedly decreased cell viability. Only samples with very low silver content allowed cell spreading and formation of continuous endothelial layer. Although long-term presence of this material in patient's organism would not be advantageous, antimicrobial effect of silver may be favorable for short term applications, e.g. coating of catheters. On Ti/C:H layers, the cells were similarly or more active in adhesion, proliferation and maturation than those on glass or pure amorphous carbon, respectively. This material could be suitable for long-term use in both soft and hard tissue surgery, e.g. for inner coating of vascular prostheses or surface modification of artificial bone implants.