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Ionic liquid-based microwave-assisted extraction and HPLC analysis of dehydrocavidine in corydalis saxicola Bunting

Du F.-Y, Xiao X.-H, Xu P. –P, Li G. –K

Acta Chromatographica

2010

Vol. 22, no. 3

459--471

An efficient ionic liquid-based microwave-assisted (IL-MAE) method has been developed for extraction of dehydrocavidine from Corydalis saxicola Bunting (C. saxicola) for subsequent rapid analysis by high-performance liquid chromatography (HPLC). The yield of dehydrocavidine reached 9.446 mg g -1 within 10 min under the optimum IL-MAE conditions (1.5 mol L -1 [hmim]Br as extraction solvent, liquid-to-solid ratio 20:1 (mL:g), and extraction temperature 70°C). Compared with conventional procedures, the proposed IL-MAE method has many advantages, for example high extraction yield, short extraction time, low solvent consumption, no use of volatile organic solvents, and no further sample clean-up before HPLC analysis. The method was validated for limit of detection (LOD) and quantification (LOQ), linearity, precision, recovery, and reproducibility. The calibration range was 5.0–200 mg L -1 and the correlation coefficient, r , was 0.9996. The LOD and LOQ were 0.035 and 0.12 mg L -1, respectively. The relative standard deviations of intra-day and inter-day assays were below 2.6% and 6.5%, respectively. Recovery was between 93.8% and 109.3% with RSD values below 5.0%. The method can be used for rapid and effective extraction and analysis of active components from medicinal plants.