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Techniki mikroskopowe w badaniach biologicznych

Bartoszek N., Rosowski M.

Laboratorium - Przegląd Ogólnopolski

2017

nr 9-10

12--21

Opracowanie prezentuje przykładowe wykorzystanie różnych technik mikroskopowych do obrazowania obiektów biologicznych. W przykładach zobrazowano komórki bakterii oraz komórki zwierzęce, a także ludzkie płytki krwi oraz nicienia Ceanorhabditis elegans. Do uzyskania obrazów wykorzystano techniki mikroskopii optycznej, w tym fluorescencyjnej, a także skaningowej mikroskopii elektronowej, transmisyjnej mikroskopii elektronowej oraz mikroskopii sił atomowych.

The paper presents an exemplary use of various microscopic techniques for the imaging of biological objects. The examples show bacterial and animal cells, as well as human blood platelets and nematode Ceanorhabditis elegans. Optical imaging techniques, including fluorescence imaging, as well as scanning electron microscopy, transmission electron microscopy and atomic force microscopy have been used to obtain the images.

The influence of the chain length and the functional group steric accessibility of thiols on the phase transfer efficiency of gold nanoparticles from water to toluene

Soliwoda K., Tomaszewska E., Tkacz-Szczesna B., Rosowski M., Celichowski G., Grobelny J.

Polish Journal of Chemical Technology

2014

Vol. 16, nr 1

86--91

This paper describes the influence of the chain length and the functional group steric accessibility of thiols modifiers on the phase transfer process efficiency of water synthesized gold nanoparticles (AuNPs) to toluene. The following thiols were tested: 1-decanethiol, 1,1-dimethyldecanethiol, 1-dodecanethiol, 1-tetradecanethiol and 1-oktadecanethiol. Nanoparticles (NPs) synthesized in water were precisely characterized before the phase transfer process using Atomic Force Microscopy (AFM) and Transmission Electron Microscopy (TEM). The optical properties of AuNPs before and after the phase transfer were studied by the UV-Vis spectroscopy. Additionally, the particle size and size distribution before and after the phase transfer of nanoparticles were investigated using Dynamic Light Scattering (DLS). It turned out that the modification of NPs surface was not effective in the case of 1,1-dimethyldecanethiol, probably because of the difficult steric accessibility of the thiol functional group to NPs surface. Consequently, the effective phase transfer of AuNPs from water to toluene did not occur. In toluene the most stable were nanoparticles modified with 1-decanethiol, 1-dodecanethiol and 1-tetradecanethiol.