Wyniki wyszukiwania - BazTech

1

Wpływ źródła witamin na produkcję erytrytolu z glicerolu przez drożdże Yarrowia lipolytica

Tomaszewska L., Cybulski K., Rywińska A.

Inżynieria i Aparatura Chemiczna

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2012

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Nr 4

184-186

PL

Celem pracy była ocena wpływu różnych źródeł witamin - ekstraktu drożdżowego, namoku kukurydzianego oraz tiaminy, na wydajność i dynamikę produkcji erytrytolu z glicerolu przez szczep Y. lipolytica Wratislavia K1. Najlepsze wyniki uzyskano przy zastosowaniu 1 g•dm-3 ekstraktu drożdżowego. W okresowej hodowli bioreaktorowej drożdże produkowały 42,2 g•dm-3 erytrytolu z wydajnością 0,43 g•g-1 i szybkością produkcji 0,63 g•dm-3 •h-1.

EN

The aim of this study was to examine the impact of different vitamins sources, such as yeast extract, corn steep liquor and thiamine, on yield and dynamics of erythritol production from glycerol by strain Y. lipolytica Wratislavia K1. The best results were achieved using 1 g•dm-3 of yeast extract. In a batch bioreactor culture yeast produced 42.2 g•dm-3 of erythritol with 0.43 g•g-1 yield and volumetric productivity of 0.63 g•dm-3 •hr-1.

2

Biosynteza erytrytolu z glicerolu przez Yarrowia lipolytica Wratislavia 1.31

Rywińska A., Tomaszewska L., Marcinkiewicz M., Rymowicz W.

Inżynieria i Aparatura Chemiczna

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2012

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Nr 4

179-181

PL

Celem pracy było zbadanie wpływu NaCl na biosyntezę erytrytolu z glicerolu przez szczep Y. lipolytica Wratislavia 1.31. W hodowlach wstrząsanych zbadano wpływ stężenia NaCl, w zakresie 0 - 8%. Najlepsze wyniki uzyskano przy 2,5% dodatku NaCl. W hodowlach bioreaktorowych, przy wykorzystaniu glicerolu odpadowego, stężenie erytrytolu wynosiło 67,5 g•dm-3, a wydajność i szybkość objętościowa jego produkcji odpowiednio 0,46 g•g-1, i 0,68 g•dm-3h-1.

EN

The aim of this study was to examine the effect of NaCl on erythritol production from glycerol by Wratislavia 1.31 strain of Y. lipolytica. The effect of NaCl for concentration ranging from 0% to 8%, was investigated shake-flasks experiment. The best results were achieved at 2.5% of NaCl. In crude glycerol-containing media the erythritol production of 67.5 g•dm-3, corresponding to 0.28 g•g-1, yield and the productivity of 0.68 g•dm-3hr-1 was achieved.

3

Peptone as a nitrogen source for erythritol production from glycerol by Yarrowia lipolytica yeast

Tomaszewska L.

Challenges of Modern Technology

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2012

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Vol. 3, no. 4

49--52

EN

Erythritol is a compound widely distributed in nature. It found application in medicine, cosmetics, chemical and food industry. It has 60–80% sweetness in comparison to sucrose, very low energy value (~0.2 kcal/g), is non-cariogenic and free of gastric side-eff ects. This sugar alcohol is commercially produced in microbiological processes using glucose and sucrose. Glycerol, which is produced in large amounts by biodiesel industry, can be used as alternative substrate for the production of erythritol by Yarrowia lipolytica yeast. The aim of the study was to examine the impact of peptone on erythritol production from glycerol by Wratislavia K1 strain of Y. lipolytica. In the 10-days shake-fl asks experiment the peptone concentration of 1–12 g/L were examined. Pure glycerol (98% wt/wt) was used as carbon and energy source. The media were supplemented with 2.5% and 5% of NaCl. The results showed that peptone could be used as nitrogen source in erythritol biosynthesis from glycerol by Y. lipolytica yeast. The best results were achieved with 2 g/L of peptone and 5% of NaCl, where yeast produced 18.2 g/L of erythritol, corresponding to 0.23 g/g yield, 0.11 g/(Lh) volumetric productivity and specifi c production rate of 0.010 g/(gh). In this conditions minimal level of of by-products was formed — arabitol production was not observed while mannitol, citric acids and α-ketoglutaric acid did not exceed 0.4, 4.4 and 2.0 g/L, respectively.