A microbial fuel cell (MFC) is a bioelectrochemical reactor in which microorganisms, feeding on organic matter, generate electricity. In such a reactor, microorganisms active on the anode form a biofilm, whose activity is a key factor determining the performance of the MFC. Biofilm also forms in water transfer installations, in substrate transfer installations in biogas plants, etc. However, in such cases such biofilm can be a source of microbiological infections or corrosion. In addition, such biofilm is composed of various microorganisms, not necessarily producing electrons. In the case of biofilm formed on the electrode in MFC, the most important thing is to build a thin layer of biofilm from electron-producing microorganisms. This paper discusses the theoretical part of biofilm (bio-catalyst) formation and carries out the procedure of building a biofilm on a carbon electrode. It has been shown that to obtain a biofilm capable of generating electricity, at least three start-ups are necessary before the electrode reaches the appropriate operating parameters. After obtaining a ready-to-use electrode with an active biofilm, measurements of electricity generation in the MFC were carried out. The results demonstrated the effectiveness of performing multiple startups to achieve a suitable working electrode with an active biofilm.
Due to the constant growth of the world's population, the amount of generated wastewater is also constantly increasing. One of the devices that can use wastewater as a raw material for energy production is a microbial fuel cell (MFC). MFCs technology is constantly evolving. However, to increase its use, it is necessary to improve its efficiency. There are various possibilities to ensure this, such as the use of new electrode materials, new cell designs, or the use of wastewaters from different sources. In this paper the analysis of MFC operation (cell voltage, power, and current density) fed by mixed municipal and industrial wastewaters was shown. Moreover, the change in time of COD was analyzed. Due to cost reduction the membrane-less microbial fuel cell (ML-MFC) was chosen. It was noted that the addition of concentrated process wastewater increases the COD reduction time in the ML MFC. An increase of generated bioelectricity during fed ML-MFC by mixed municipal and industrial (process wastewater from yeast production) wastewater was demonstrated. The highest values of average cell voltage (598 mV), maximum power (4.47 mW) and maximum current density (0.26 mA•cm-2) were obtained for a 10% share of yeast process wastewater in the mixed wastewater, which fed the ML-MFC.
Wastewater has high potential as an energy source. Therefore, it is important to recover even the smallest part of this energy, e.g., in microbial fuel cells (MFCs). The obtained electricity production depends on the process rate of the electrodes. In MFC, the microorganisms are the catalyst of anode, and the cathode is usually made of carbon material. To increase the MFC efficiency it is necessary to search the new cathode materials. In this work, the electricity production from yeast wastewater in membrane-less microbial fuel cells with a carbon felt, Ni-Co, Cu-B, and Cu-Ag cathodes has been analyzed. In the first place, the measurements of the stationary potential of the electrodes (with Cu-Ag catalyst obtained by the electrochemical deposition technique) were performed. Next, the analysis of the electric energy production during the operation of the membrane-less microbial fuel cell (ML-MFC). The highest parameters were obtained for the Ni-Co and Cu-Ag catalysts. The cell voltage of 607 mV for Ni-Co and 605 mV for Cu-Ag was obtained. Additionally, the power of 4.29 mW for both cathodes - Ni-Co and Cu-Ag was obtained. Moreover, Ni-Co and Cu-Ag allow the shortest time of COD reduction. Based on the test results (with selected MFC design, wastewater, temperature, etc.), it can be concluded that of all the analyzed electrodes, Cu-Ag and Ni-Co electrodes have the best parameters for use as cathodes in ML-MFC. However, based on the results of this study, it can be concluded that all the tested electrodes can be used as cathode material in MFC.
The possibility to combine wastewater treatment and electricity production can accomplish a microbial fuel cell. Microbial fuel cells use glucose from wastewater as a fuel. In recent years, both production of municipal and industry wastewater increases very much. Municipal wastewater is directed to the wastewater treatment plant. While industry wastewater can be use as a fertilizer. But, both municipal and industry wastewater can be used in the microbial fuel cells. The comparison of powering the microbial fuel cell with municipal and process wastewater from yeast production is presented in this paper. The measurements covered comparison of changes in the concentration of COD in the reactor without aeration, with aeration and with using a microbial fuel cell (powered with municipal and industry wastewater). The results of measurements of COD showed no differences between the microbial fuel cell powered with municipal wastewater and the microbial fuel cell powered with process yeast wastewater. But, the power output is higher with using process yeast wastewater to powering the microbial fuel cell.
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