The introduction of the subsequent European emission standards raises controversy among vehicle manufacturers and researchers. In order to meet the legal requirements, it is necessary to continually improve not only the cars but also the costly measurement facilities. In addition, current methods do not give a clear answer to the harmful effects of exhaust gases on living organisms. By using the appropriate In vitro method to directly assess the toxicity of gaseous mixtures, one can obtain simple, reliable results that can be used to validate the introduction of ever more stringent Euro standards.
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