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E-beam irradiation for the control of Phytophthora nicotianae var. nicotianae in stonewool cubes

Ptaszek M., Orlikowski L. B., Migdał W., Gryczka U.

Nukleonika

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2015

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Vol. 60, No. 3, part 2

679--682

EN

Effectiveness of electron beam irradiation was evaluated against Phytophthora nicotianae var. nicotianae, the causal agent of stem base and root rot of tomato. In laboratory trials, irradiation of 7-day-old Phytophthora cultures growing on potato-dextrose-agar (PDA) medium with 1 kGy resulted in the disintegration of the pathogen’s hyphae. Increasing the irradiation dose to 3 kGy caused decay of the hyphae. Irradiation of infested stonewool with 5 kGy caused decrease of the pathogen population about 5 times. Application of 20 kGy completely eliminated the pathogen from stonewool. Irradiation of substratum resulted in significant increase of tomato seedlings healthiness, especially when the dose 20 kGy was applied.

2

Technologia radiacyjna w ochronie roślin

Gryczka U., Migdał W., Ptaszek M., Orlikowski L. B.

Postępy Techniki Jądrowej

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2014

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z. 2

23--26

3

Radiation methods in decision support system for food safety

Migdal W., Gryczka U., Bertrandt J., Nowicki T., Pytlak R.

Nukleonika

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2014

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Vol. 59, No. 4

161--168

EN

Mathematical model is the basis for computer decision support system (DSS) connected with food security strategy, in which food irradiation is one of the methods used for elimination of substance with pathogens. Such system can support an organisation of many different activities in the area of food safety. It can be designed for a specifi c region, country, etc., and is addressed to State Sanitary Inspection offi ces. The paper demonstrates the capabilities of the system in modeling activities of sanitary inspection teams aimed at pathogen elimination with the use of electron beam irradiation in procedures of utilisation of animal originated food, related packaging, or other microbiologically contaminated materials. It describes new application of both computer supported decision systems and radiation processing. The elaborated mathematical models show human activities in the process of microbiological contamination elimination.

4

Radiacyjna metoda higienizacji i utrwalania żywności

Migdał W., Gryczka U.

Postępy Techniki Jądrowej

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2013

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z. 1

8--14

5

Effectiveness of electron beam irradiation in the control of Rhizoctonia solani

Orlikowski L. B., Gryczka U., Ptaszek M., Migdał W.

Nukleonika

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2012

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Vol. 57, No. 4

551-554

EN

Effectiveness of electron beam irradiation was evaluated against Rhizoctonia solani, which is one of the most dangerous soil-borne pathogen, causing stem base and root rot of many horticultural plants. Treatment of in vitro cultures with 0.5 kGy significantly inhibited the pathogen's growth and the spread of necrosis on chrysanthemum leaf blades inoculated with the irradiated cultures. Application of e-beam irradiation for peat disinfection resulted in a two-fold decrease rot development in chrysanthemum stem at a dose of 5 kGy and complete inhibition at 10 kGy. For the elimination of R. solani from composted pine bark and its mixture with peat, irradiation of both substrates with 15 kGy was necessary.

6

Effectiveness of electron beam irradiation in the control of some soilborne pathogens

Orlikowski L. B., Migdał W., Ptaszek M., Gryczka U.

Nukleonika

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2011

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Vol. 56, No. 4

357-362

EN

Electron beam (EB) irradiation was tested against Botrytis cinerea, Pythium ultimum and Phytophthora citricola the most dangerous pathogens causing stem and root rot of seedlings, cuttings and older plants. In the laboratory trials cultures of 3 species were irradiated with doses 0 (control), 1.5, 3.0, 4.5 and 6.0 kGy whereas peat was treated with 10,15 and 25 kGy. P. citricola was the most sensitive species for irradiation. In greenhouse trials 15 kGy irradiation of peat protected chrysanthemum cuttings against B. cinerea and P. ultimum as well as rhododendron young plants against P. citricola. Irradiation of peat did not influence the growth and development of the tested plants.

7

Application of electron beam irradiation for inhibition of Fusarium oxysporum f. sp. dianthi activity

Gryczka U., Ptaszek M., Migdał W.

Nukleonika

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2010

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Vol. 55, No. 3

359-362

EN

Electron beam irradiation was tested against Fusarium oxysporum f. sp. dianthi (Fod) a pathogen causing Fusarium wilt of carnation. Efficiency of the different radiation doses on in vitro survival and development of Fod culture on potato-dextrose agar (PDA) medium was tested. A dose of 6 kGy completely inhibited the pathogen growth. Application of radiation for microbiological decontamination of four substrates used for carnation production demonstrated that, depending on the type of substrate, doses of 10 or 25 kGy were effective in Fod elimination. All carnation plants cultivated on radiation decontaminated substrates were healthy.

8

Application of dose reader CD-07 for depth-dose distribution measurements in the process of microbiological radiation decontamination

Gryczka U., Jakowiuk A., Migdał W., Kałuska I., Machaj B.

Nukleonika

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2009

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Vol. 54, No. 4

261-265

EN

Dose reader CD-07 has been introduced for measurements of a dose distribution during dose mapping exercises and for calculations of electron beam energy. This instrument consists of an SP-880 spectrophotometer equipped with a driving motor to move a dosimetric foil across light beam in the front of narrow light aperture. To control the movement of the foil and to present and store the measuring results a specialized software has been developed. In this paper the use of dose reader CD-07 in Performance Qualification of irradiated herbs was described.

9

Study on biological activity of chitosan after radiation processing

Gryczka U., Gawrońska A., Migdał W, Gawroński S. W., Chmielewski A. G.

Nukleonika

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2008

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Vol. 53, suppl. 2

73--76

EN

In recent years there is a trend in industry to limit the usage of chemical compounds. Natural polymers are new promising materials that possess important properties like biodegrability or lack of toxicity. Radiation processing of natural occurring polymers is an area of current research for development of new applications. The aim of this study was to determine the impact of ionizing radiation modification on bioactivity of a natural polysaccharide. Chitosan with different molecular weights was investigated as a biostimulator – a biologically active substance that stimulates some growth processes in plants. Chitosan in solid state was irradiated with electron beam from an electron beam accelerator Elektronika 10-10 with a dose range from 50 to 300 kGy. The effects of irradiation on the molecular weight of chitosan were investigated by viscosity and GPC measurements. Non-irradiated and irradiated chitosan at concentrations 0.001, 0.01, 0.1 and 1 g/dm3 were used for greenhouse tests of its activity for growth promotion of Salix viminalis L. var. gigantea plant. Uniform rooted cuttings (20 per combination) were selected for the test and cultivated in aerated hydroponics culture containing Hoagland’s nutrient solutions plus respective amounts of chitosan. After six weeks of plant exposure to chitosan, data of selected parameters of plant growth were collected. In most cases, except the highest concentration, both forms of chitosan had stimulatory effect on leaf area, length of roots and of newly developed shoots. Also fresh and dry weights of these organs were greater in chitosan treated plants. The highest concentration of chitosan was stimulatory only for a number of roots and newly developed shoots while for other parameters was inhibitory. In comparable concentrations the stimulatory effect was greater for chitosan irradiated in comparison with the non-irradiated one.