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Comparison of Matrix Solid-Phase Dispersion and Liquid—Solid Extraction Methods Followed by Solid-Phase Extraction in the Analysis of Selected Furanocoumarins from Pimpinella Roots by HPLC—DAD

Oniszczuk A., Waksmundzka-Hajnos M., Podgórski R., Nowak R., Żukiewicz-Sobczak W., Blicharski T.

Acta Chromatographica

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2015

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Vol. 27, no. 4

687--696

EN

The use of matrix solid-phase dispersion (MSPD) was tested to extract some coumarins (umbelliferone, xanthotoxin, isopimpinellin, bergapten, and pimpinellin) from Pimpinella roots. The method was compared with liquid—solid extraction methods (LSE) such as accelerated solvent extraction (ASE), ultrasound-assisted extraction (USAE) and Soxhlet extraction followed by solid-phase extraction (SPE). Several methanol concentrations and temperature conditions were examined during the liquid—solid extraction techniques. The analysis was performed by high-performance liquid chromatography with diode array detector (HPLC—DAD). MSPD extracted furanocoumarins with satisfactory recoveries ranging from 91.65% to 97.55% and relative standard deviations lower than 5,0415%. The results presented in the paper reveal that MSPD is an efficient, fast, simple and easy-to-perform method suitable for the isolation of furanocoumarins from herbs.

2

Influence of sample preparation methods on the quantitation of selected tropane alkaloids from herb of Datura innoxia mill. by HPTLC

Oniszczuk A., Waksmundzka-Hajnos M., Gadzikowska M., Podgórski R., Oniszczuk T.

Acta Chromatographica

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2013

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Vol. 25, no. 3

545--554

EN

The aim of our paper was the examination of various procedures of extraction and purification methods on the yield of atropine and scopolamine in Datura innoxia extracts determined by high-performance thin-layer chromatography (HPTLC). In our experiments, the following extraction methods were used: percolation, ultrasound-assisted extraction (USAE) at various temperatures, and microwave-assisted extraction (MASE). The highest extraction yields of atropine and scopolamine were obtained by ultrasound-assisted extraction method and aqueous acetic acid or aqueous tartaric acid used as extractants. Crude extracts of D. innoxia herb were purified by use of various methods: solid-phase extraction-strong cation exchanger (SPE-SCX), SPE-C18, and liquid-liquid extraction (LLE). The best results of purification with satisfactory %RSD values were obtained by liquid-liquid extraction by use of dichloromethane from aqueous crude alkaloid extract alkalized to pH 12. HPTLC was performed on pre-coated plates Kieselgel 60 F254 in horizontal DS chambers by use of mobile phase: methanol-acetone-aqueous ammonia (5:4.5:0.5). The alkaloids’ content was determined in extracts by use of calibration curve method for atropine and scopolamine. Calibration curves were linear in range of 3.0–30 μg for scopolamine (R = 0.9980) and 2.6–26 μg for atropine (R = 0.9923). Limits of detection (LOD) and limits of quantification (LOQ) were 1.12 μg and 3.42 μg per spot for scopolamine and 0.89 μg and 2.82 μg per spot for atropine.

3

Wpływ rodzaju i parametrów ekstrakcji na wydajność izolacji wybranych furanokumaryn z owoców gorysza wyniosłego (Peucedanum verticillare)

Waksmundzka-Hajnos M., Oniszczuk A., Podgórski R.

Camera Separatoria

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2012

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Vol. 4, No. 1

83--91

PL

Analiza materiału roślinnego jest ważnym zadaniem w poszukiwaniu roślin o działaniu farmakologicznym a także w standaryzacji leków roślinnych. Celem każdego procesu ekstrakcji jest szybka i skuteczna izolacja związków z matrycy przy użyciu minimalnej ilości rozpuszczalnika. Celem przedstawionej pracy był wybór optymalnych warunków do analizy materiału roślinnego oraz zbadanie wpływu metody ekstrakcji na wydajność izolacji niektórych furanokumaryn z owoców gorysza wyniosłego. Furanokumaryny mają istotne zastosowanie w lecznictwie. Są między innymi wykorzystywane w terapii bielactwa i łuszczycy oraz leczeniu skórnego T-komórkowego chłoniaka z erytmodermią. Zastosowano następujące metody ekstrakcyjne: wyczerpująca ekstrakcja w aparacie Soxhleta, ekstrakcja wspomagana ultradźwiękami w temperaturze 20 i 60◦C oraz ekstrakcja wspomagana mikrofalami w układzie otwartym i zamkniętym. Analiza została przeprowadzona metodą wysokosprawnej chromatografii cieczowej z detektorem UV/VIS. Wyniki wskazują, że najwyższą wydajność imperatoryny uzyskano w wyniku ekstrakcji ultradźwiękowej 60◦C, natomiast metodą najskuteczniejszą do izolacji umbeliferonu okazała się ekstrakcja mikrofalowa w układzie zamkniętym.

EN

Analysis of plant material is an important task in chemotaxonomical investigations, particularly, in search of plants with pharmacological activity or in standardisation of plant drugs. The goal of every extraction process is rapid and effective isolation of compounds from a matrix by use of minimum amount of solvent. The aim of this paper was the choice of optimal condi-tions for the analysis of plant material and effect of extraction method on the yield of some furanocoumarins from Peucedanum verticillare fruits. Furanocoumarins have important uses in human medicine and exhibit toxicity against a wide range of organisms. This group of com-pounds reveal pharmacological activity. They are important drugs in vitiligo and psoriaris therapy and are also used in therapy erythrodermic variants of cutaneous T-cell lymphoma and chronic graft-versus-host disease. The following extraction methods were used in our experiments: exhaustive extraction in Soxh-let apparatus, ultrasound- assisted solvent extraction (USAE) at 20 and 60◦C as well as micro-wave-assisted solvent extraction in open and closed system (MASE). Target compounds analysis was performed by high performance liquid chromatography with UV/VIS detector. The results indicated that, the highest yield of imperatorin can be obtained by ultrasonification at 60◦C, while microwave-assisted solvent extraction in closed system is very efficient in the extraction of umbelliferone.