Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 2

Liczba wyników na stronie
first rewind previous Strona / 1 next fast forward last
Wyniki wyszukiwania
help Sortuj według:

help Ogranicz wyniki do:
first rewind previous Strona / 1 next fast forward last
EN
A liquid chromatographic fingerprinting methodology has been established for identification and quality control of traditional herbal medicines. The methodology was developed from four case studies. Samples of Herba Artemisia annua, Herba Artemisia scoparia, Rhizoma Ligusticum chuanxiong (also called Rhizoma chuanxiong ), and Rhizoma Ligusticum jeholense (also called Rhizoma ligustici ) were investigated. In each case study, sample preparation and chromatographic conditions (column, organic modifier, column temperature, detection wavelength, and mechanism) were varied to obtain good fingerprints, i.e. with the maximum number of peaks. Further optimization was then performed either by reducing the analysis time or increasing efficiency. The case studies led to the development of a general methodology consisting of sample preparation and HPLC fingerprint development. The fingerprints obtained with the developed methodology were then successfully used to distinguish between the two Artemisia species and between the two Ligusticum species. The methodology also was used to obtain fingerprints for a large number of different Vietnamese Mallotus species, and for Citri reticulatae pericarpium samples collected in different regions of China.
2
Content available remote The use of experimental design in separation science
EN
In this tutorial, the application of experimental designs in separation science is discussed. Method optimization is often divided into screening and optimization phases. In the screening step, many factors, potentially affecting the separation, are screened to identify those with the largest effects. These are then further examined in an optimization phase, to determine the best separation conditions. After optimizing the method, it should be validated, before use for quantitative purposes. Robustness testing is part of method validation and examines the effects on the responses of small changes in method conditions. During the first phase of method optimization and during robustness testing, so-called screening designs are usually applied, whereas during the second phase of optimization, response-surface designs are often used. In this tutorial, the different steps in the application of both types of design are explained and elaborated with some examples.
first rewind previous Strona / 1 next fast forward last
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.