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Radiological evaluation of mandibular alveolar reconstruction after retained third molar removal procedures using autogenous dentin matrix (ADM)

Kubaszek Bartłomiej, Kiełboń Agnieszka, Kasprzyk-Kucewicz Teresa, Starosta Martin, Krynicki Paweł, Morawiec Tadeusz

Polish Journal of Medical Physics and Engineering

2024

Vol. 30, Iss. 2

90--98

Introduction: Bone regeneration materials are often used in dental surgery procedures. At present, we have the possibility to use materials from different groups due to the way they are obtained. The gold standard still remains the use of autogenous bone substitute material due to its osteogenic, osteoinductive and osteoconductive properties. The surgical removal of the third retained tooth is one of the most common procedures performed in dental surgery. As a result of such a procedure, bone tissue atrophy occurs. In order to avoid the formation of a bone defect on the distal surface of the second molar, an augmentation method using an autogenous dentin matrix can be used in such procedures. Material and methods: Procedures for bilateral removal of retained third molars were performed in 15 patients. On one side, augmentation was performed with an autogenous dentin matrix obtained by grinding and preparing the extracted retained tooth (study group). On the opposite side, the bone defect was left to heal spontaneously (control group). After 6 months, the degree of bone regeneration was compared on the basis of bone density testing and the size of the resulting bone defect on the distal side was compared on the basis of radiographic examination. Results: Analysis of the obtained material showed significant differences in healing after treatments with autogenous dentin matrix. Thus, the use of an autogenous dentin matrix made it possible to obtain a completely regenerated bone defect. Conclusions: This procedure seems to be useful for bone regeneration after the removal of the third molar.

Evaluation of changes caused by HR-HPV infection in squamous cell carcinoma of the head and neck using Raman microspectroscopy in combination with multivariate statistical analysis

Szurko Agnieszka, Zygadło Dorota, Student Sebastian, Śnietura Mirosław, Lorenc Zbigniew, Kiełboń Agnieszka, Waniczek Dariusz

Polish Journal of Medical Physics and Engineering

2024

Vol. 30, Iss. 2

69--78

Introduction: Squamous cell carcinoma of the head and neck region (HNSCC), with a positive status for high oncogenic potential human papillomavirus (HR-HPV), represents a clinically distinct disease entity compared to HPV-independent cases. Patients exhibit variations in prognosis and proposed therapy regimens. A prompt and reliable diagnosis of the presence of HPV infection could optimize the treatment for these patients. Currently employed treatment methods are long-term, expensive, and lack specificity, especially when administered separately. Material and methods: The research objective of this study is to explore significant differences in the Raman spectra of biological samples taken from patients with HNSCC, facilitating the confirmation of HPV virus presence. Study groups were delineated based on histopathological diagnosis and molecular biology tests, confirming the biological activity of the virus and the presence of the HR-HPV form with a diagnosis of a specific subtype. Results: To identify high oncogenic potential human papillomavirus (HR-HPV) infection as a crucial factor in squamous cell carcinoma of the head and neck region, an effective automatic data analysis system was established, relying on Raman microspectroscopy and multivariate analysis. Our results showed clear ranges of the Raman spectrum that differentiated between HPV-associated and non-HPV-associated cancers. Conclusions: In conclusion, our experience shows a great diagnostic potential of Raman confocal microscopy with multidimensional statistical analysis. In the future, the use of this method may allow for the creation of an effective and automated HR-HPV detection system in neoplastic tissue.

Wpływ wstępnego schłodzenia ciała z wykorzystaniem automatycznego nawiewu na wartość diagnostyczną obrazów termicznych gruczołu piersiowego

Szurko Agnieszka, Kiełboń Agnieszka, Lasyk Dorota, Kokoszka Katarzyna, Stanek Agata, Cholewka Armand

Inżynier i Fizyk Medyczny

2022

Vol. 11, nr 6

473--478

Termografia, jako nieinwazyjna metoda pozwalająca szybko i skutecznie wykryć obszary o podwyższonej temperaturze powierzchni ciała, idealnie nadaje się do celów wspomagających diagnostykę schorzeń piersi. Celem przeprowadzonych badań było udoskonalenie procedur badawczych z wykorzystaniem termowizji poprzez zastosowanie wstępnego schłodzenia ciała ochotników. Pomiary wykonano w Laboratorium Medycyny Sportowej Instytutu Inżynierii Biomedycznej na Uniwersytecie Śląskim. Grupa badawcza składała się z 5 zdrowych, młodych osób. Ciało ochotników zostało schłodzone w komorze kriogenicznej CrioSpace firmy JBG2 przy pomocy powietrza o temperaturze 0°C. Na uzyskanych termogramach gruczołów piersiowych oznaczone zostały obszary pomiarowe według stosowanej w medycynie konwencji. Analizowane termogramy oraz parametry temperaturowe jednoznacznie wykazały zwiększenie zakresu obserwowanych różnic temperaturowych po schłodzeniu piersi. Kontrast temperaturowy obliczany na podstawie różnic średnich temperatur symetrycznych obszarów piersi wzrastał nawet kilkakrotnie. Prosty zabieg ochłodzenia badanego obszaru może zatem podnieść czułość i dokładność pomiarów termowizyjnych.

Thermography as a non-invasive method that allows to detect quickly and effectively areas with increased body surface temperature, is ideally suited for supporting the diagnosis of breast diseases. The aim of the research was to improve research procedures using thermovision by applying pre-cooling of the volunteers’ bodies. The measurements were made at the Sports Medicine Laboratory of the Institute of Biomedical Engineering at the University of Silesia. The research group consisted of 5 healthy young people. The body of the volunteers was cooled in the CryoSpace cryogenic chamber by JBG2, with the use of air at a temperature of 0℃. Measurement areas were marked on the obtained thermographs of the mammary glands according to the convention used in medicine. The analysed thermograms and temperature parameters clearly showed an increase in the range of observed temperature differences after breast cooling. The temperature contrast, calculated on the basis of differences in mean temperatures of symmetrical breast areas, increased even several times. A simple treatment of cooling the examined area can therefore increase the sensitivity and accuracy of thermal imaging measurements.